CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

Nasir Javaid; Sangdun Choi

doi:10.3389/fcell.2021.761709

CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

Front Cell Dev Biol. 2021 Nov 24:9:761709. doi: 10.3389/fcell.2021.761709. eCollection 2021.

Authors

Nasir Javaid¹, Sangdun Choi^{1

2}

Affiliations

¹ Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
² S&K Therapeutics, Ajou University Campus Plaza, Suwon, South Korea.

Abstract

The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.

Keywords: CRISPR/cas system; classification; epigenome; genome-editing efficiency; host DNA repair.

Publication types

Review