A Novel Conductive Antibacterial Nanocomposite Hydrogel Dressing for Healing of Severely Infected Wounds

Lizhi Xiao; Fang Hui; Tenghui Tian; Ruyue Yan; Jingwei Xin; Xinyu Zhao; Yingnan Jiang; Zhe Zhang; Yulan Kuang; Na Li; Yu Zhao; Quan Lin

doi:10.3389/fchem.2021.787886

A Novel Conductive Antibacterial Nanocomposite Hydrogel Dressing for Healing of Severely Infected Wounds

Front Chem. 2021 Nov 24:9:787886. doi: 10.3389/fchem.2021.787886. eCollection 2021.

Authors

Lizhi Xiao¹, Fang Hui¹, Tenghui Tian¹, Ruyue Yan¹, Jingwei Xin², Xinyu Zhao¹, Yingnan Jiang¹, Zhe Zhang¹, Yulan Kuang¹, Na Li³, Yu Zhao¹, Quan Lin²

Affiliations

¹ Jilin Ginseng Academy, Hospital of Affiliated Changchun University of Chinese Medicine, Changchun University of Chinese Medicine, Changchun, China.
² State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Chinese-Japan Union Hospital of Jilin University, Jilin University, Changchun, China.
³ Key Laboratory of Songliao Aquatic Environment of Ministry of Education, Jilin Jianzhu University, Changchun, China.

Abstract

Wound infections are serious medical complications that can endanger human health. Latest researches show that conductive composite materials may make endogenous/exogenous electrical stimulation more effective, guide/comb cell migration to the wound, and subsequently promote wound healing. To accelerate infected wound healing, a novel medical silver nanoparticle-doped conductive polymer-based hydrogel system (Ag NPs/CPH) dressing with good conductivity, biocompatibility, and mechanical and antibacterial properties was fabricated. For the hydrogel dressing, Ag NPs/CPH, polyvinyl alcohol (PVA), and gelatin were used as the host matrix materials, and phytic acid (PA) was used as the cross-linking agent to introduce conductive polyaniline into the matrix, with antibacterial Ag NPs loaded via impregnation. After a series of analyses, the material containing 5 wt% of PVA by concentration, 1.5 wt% gelatin, 600 μL of AN reactive volume, and 600 μL of PA reactive volume was chosen for Ag NPs/CPH preparation. XPS and FTIR analysis had been further used to characterize the composition of the prepared Ag NPs/CPH. The test on the swelling property showed that the hydrogels had abundant pores with good water absorption (≈140% within 12 h). They can be loaded and continuously release Ag NPs. Thus, the prepared Ag NPs/CPH showed excellent antibacterial property with increasing duration of immersion of Ag NPs. Additionally, to evaluate in vivo safety, CCK-8 experiments of HaCat, LO2 and 293T cells were treated with different concentrations of the Ag NPs/CPH hydrogel soaking solution. The experimental results showed the Ag NPs/CPH had no significant inhibitory effect on any of the cells. Finally, an innovative infection and inflammation model was designed to evaluate the prepared Ag NPs/CPH hydrogel dressing for the treatment of severely infected wounds. The results showed that even when infected with bacteria for long periods of time (more than 20 h), the proposed conductive antibacterial hydrogel could treat severely infected wounds.

Keywords: Ag nanoparticles; bacteriostatic medical hydrogel; conductivity; infected wound healing; new animal model.