Promotion of methane production by magnetite via increasing acetogenesis revealed by metagenome-assembled genomes

Abstract

Metal oxides are wildly studied to enhance anaerobic digestion and the methanogenic process, which is generally interpreted by increased direct interspecies electron transfer (DIET). Yet microbial mechanisms involved are under debate. Herein, methane production dynamics were analyzed, and acceleration on biogas accumulation was presented. Complementing previous findings, Fe₃O₄ nanoparticles stimulated bacterial fermentation rather than methanogenesis or syntropy between electro-microorganism and methanogen. More importantly, metagenome-assembled genomes proved that Fe₃O₄ nanoparticles increased acetogenesis by Parabacteroides chartae, which provided abundant substrates for acetoclastic methanogenesis. Interestingly, the weakly conductive V₃O₇·H₂O nanowires increased potential hydrogen-producing bacteria, Brevundimonas, and electro-microorganisms, Clostridium and Rhodoferax, which is convenient for conducting DIET. Collectively, conductivity may not be a critical factor in mediating DIET and distinct strategies of metal oxides on methane production propose more possibilities, such as fermentation process.

Keywords: Anaerobic digestion; Conductive material; Electron transfer; Fe(3)O(4) nanoparticles; Granular activated carbon; Metagenome-assembled genomes; Methane; V(3)O(7)·H(2)O nanowires.