With the development of Internet, the number of people with symptoms of Internet gaming disorder (IGD) has increased. In the past, psychologists used retrospective questionnaires to diagnose IGD. However, it is difficult to diagnose IGD symptoms instantaneously using retrospective questionnaires due to the requirement of an Internet gaming experience of greater than 6 months and the limitations of retrospective memory. Observing the physiological regulation system might instantaneously diagnose IGD. However, observation of instantaneous physiological response is limited due to the lack of appropriate techniques. Our previous study successfully combined complimentary ensemble empirical mode decomposition and normalized direct quadrature to obtain respiratory instantaneous frequency (IF) to overcome this limitation. This study uses game-related films as stimulus materials to observe the difference in respiratory IF response per second of gamers with high-risk IGD (HIGD) and low-risk IGD (LIGD). The result showed that the respiratory IF of gamers with HIGD is lower than those of gamers with LIGD at the time of stimulation. In addition, the study also observes the dynamic change in respiratory IF per second (IFdiff). The results showed that the instant at which a significant difference is observed in IFdiff between HIGD and LIGD can be matched to the stimulation of the films. In summary, this study demonstrated that the IFdiff of gamers with HIGD and LIGD are different when stimulated. Therefore, this suggests that IFdiff might be used as a potential physiological marker to instantaneously distinguish and diagnosis the risk of IGD.Clinical Relevance- This study investigates the dynamically psychophysiological response regulation by analyzing the respiratory IF of gamers diagnosed with IGD.