In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes

Kamal Hussein; Abdelbaset Eweda Abdelbaset; Ahmed Abdelrahiem Sadek; Ahmed Noreldin

doi:10.3389/fvets.2021.661426

In vitro and in vivo Effects of a Single Dose of Bupivacaine 5% on Donkey Chondrocytes

Front Vet Sci. 2021 Nov 23:8:661426. doi: 10.3389/fvets.2021.661426. eCollection 2021.

Authors

Kamal Hussein¹, Abdelbaset Eweda Abdelbaset², Ahmed Abdelrahiem Sadek¹, Ahmed Noreldin³

Affiliations

¹ Department of Animal Surgery, Anaesthesia, and Radiology, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt.
² Clinical Laboratory Diagnosis, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt.
³ Department of Histology and Cytology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.

Abstract

Single intra-articular (IA) injection of long-acting local anesthetics such as bupivacaine is commonly used clinically for postoperative analgesia, in particular, after arthroscopic surgery. Despite their widespread use, the side effects of IA bupivacaine on joint cartilage as well as hepatotoxic and nephrotoxic effects remain to be elucidated. The aim of this study is to assess the in vitro effect of bupivacaine 5% on donkey chondrocytes at different time points, in addition to the in vivo effects of a single IA bupivacaine injection on the middle carpal joint in a group of 10 clinically healthy adult male donkeys. In phase I, the effect of in vitro treatment with bupivacaine 5% or saline 0.9% on freshly isolated donkey chondrocytes for 30, 60 min, 24, 48, and 96 h was investigated using MTT and LIVE/DEAD assay. In phase II, in vivo effects of single injection of bupivacaine on the middle carpal joint of the donkey were evaluated compared with saline 0.9%. Biochemical analysis of collected serum and synovia was performed. Additionally, articular cartilage damage was evaluated using radiography, computed tomography (CT), catabolic marker expression via quantitative polymerase chain reaction (qPCR), and histopathological examination 96 h after injection. Our results showed that after a 30-min exposure to bupivacaine 5%, the viability of donkey chondrocytes was 97.3 ± 4.4% and was not significantly affected at the indicated time points (n = 8, p < 0.05). No significant changes in biochemical analytes of serum and synovial fluid following IA bupivacaine injection were observed, compared with saline injection (n = 5 for each group, p < 0.05). Furthermore, in vivo IA injection of bupivacaine revealed no significant differences in radiography, CT scan, gene expression of cartilage catabolic biomarkers, and histopathological examination. These results provide an evidence for the safety of bupivacaine on the donkey cartilage.

Keywords: bupivacaine; cartilage damage; chondrolysis; donkey; intra-articular; local anesthetic; synovial fluid.