Cassava Brown Streak Disease Response and Association With Agronomic Traits in Elite Nigerian Cassava Cultivars

Chukwuka Ugochukwu Ano; Mildred Ochwo-Ssemakula; Angele Ibanda; Alfred Ozimati; Paul Gibson; Joseph Onyeka; Damian Njoku; Chiedozie Egesi; Robert S Kawuki

doi:10.3389/fpls.2021.720532

Cassava Brown Streak Disease Response and Association With Agronomic Traits in Elite Nigerian Cassava Cultivars

Front Plant Sci. 2021 Nov 22:12:720532. doi: 10.3389/fpls.2021.720532. eCollection 2021.

Authors

Chukwuka Ugochukwu Ano^{1

2}, Mildred Ochwo-Ssemakula¹, Angele Ibanda^{1

3}, Alfred Ozimati³, Paul Gibson^{1

4}, Joseph Onyeka², Damian Njoku², Chiedozie Egesi^{2

5

6}, Robert S Kawuki³

Affiliations

¹ College of Agricultural and Environmental Sciences, Makerere University, Kampala, Uganda.
² National Root Crops Research Institute, Umudike, Nigeria.
³ National Crops Resources Research Institute, Kampala, Uganda.
⁴ Makerere University Regional Center for Crop Improvement, Kampala, Uganda.
⁵ International Institute of Tropical Agriculture, Ibadan, Nigeria.
⁶ Cornell University Root Crops Research Institute, Ithaca, NY, United States.

Abstract

Cassava mosaic geminiviruses (CMGs) and cassava brown streak viruses (CBSVs) cause the highest yield losses in cassava production in Africa. In particular, cassava brown streak disease (CBSD) is and continues to be a significant constraint to optimal cassava production in Eastern and Southern Africa. While CBSD has not been reported in West Africa, its recent rapid spread and damage to cassava productivity in Eastern, and Southern Africa is alarming. The aim of this study was to evaluate Nigerian cassava genotypes in order to determine their responses to CBSD, in the event that it invades Nigeria, the world's largest cassava producer. The study gathered information on whether useful CBSD resistance alleles are present in the elite Nigerian cassava accessions. A total of 1,980 full-sib cassava seedlings from 106 families were assessed in the field at the seedling stage for a year. A subset of 569 clones were selected and assessed for another year at the clonal stage in Namulonge, central Uganda, a known hotspot for CBSD screening. Results indicated that foliar and root incidences and severities varied significantly (p ≤ 0.01, p ≤ 0.001) except for CBSD foliar incidence at 6 months (CBSD_6i ). Highest and lowest plot-based heritability estimates for CBSD were registered for CBSD root severity (CBSD _rs ) (0.71) and CBSD_6i (0.5). Positive and highly significant correlations were noted between CBSD root incidence (CBSD _ri ) and CBSD _rs (r = 0.90^***). Significant positive correlations were also noted between CBSD foliar severity at 3 months (CBSD_3s ) and CBSD foliar incidence at 6 months (CBSD_6i ) (r = 0.77^***), CBSD_3s and CBSD _rs (r = 0.35^***). Fresh root weight (Fresh _RW ) negatively correlated with CBSD _ri and CBSD _rs , respectively (r = -0.21^*** and r = -0.22^***). Similarly, CBSD_3s correlated negatively with cassava mosaic disease severity at 3 (CMD_3s ) and 6 months (CMD_6s ), respectively (r = -0.25^*** and r = -0.21^***). Fifteen clones were selected using a non-weighted summation selection index for further screening. In conclusion, results revealed that the elite Nigerian accessions exhibited significant susceptibility to CBSD within 2 years of evaluation period. It is expected that this information will aid future breeding decisions for the improvement of CBSD resistance among the Nigerian cassava varieties.

Keywords: Ugandan cassava brown streak virus (UCBSV); breeding; cassava brown streak disease (CBSD); cassava brown streak virus (CBSV); cassava germplasm; elite genotypes; preemptive strategies; resistance screening.