The attenuation of antibiotic resistant non-albicans Candida species, cytotoxicity, anti-inflammatory effects and phytochemical profiles of five Vachellia species by FTIR and UHPLC-Q/Orbitrap/MS

Garland Kgosi More; Christinah Ramakwala Chokwe; Stephen Meddows-Taylor

doi:10.1016/j.heliyon.2021.e08425

The attenuation of antibiotic resistant non-albicans Candida species, cytotoxicity, anti-inflammatory effects and phytochemical profiles of five Vachellia species by FTIR and UHPLC-Q/Orbitrap/MS

Heliyon. 2021 Nov 19;7(11):e08425. doi: 10.1016/j.heliyon.2021.e08425. eCollection 2021 Nov.

Authors

Garland Kgosi More¹, Christinah Ramakwala Chokwe², Stephen Meddows-Taylor³

Affiliations

¹ College of Agriculture and Environmental Sciences Laboratories, University of South Africa, Florida, Johannesburg, 1710, South Africa.
² Department of Chemistry, College of Science Engineering and Technology, University of South Africa, Florida, Johannesburg, 1710, South Africa.
³ Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Florida, Johannesburg, 1710, South Africa.

Abstract

This work investigated the antifungal, cytotoxic and LPS-induced anti-inflammatory effects of five Vachellia species (V. karroo, V. kosiensis, V. sieberiana, V. tortalis and V. xanthophloea). The antifungal activity of the aqueous-methanolic extracts were performed using the broth dilution method against four non-albicans Candida species (C. glabrata, C. auris, C. tropicalis and C. parapsilosis). The cytotoxic and anti-inflammatory effects of the extracts were evaluated on African green monkey Vero kidney cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and the 2',7'-dichlorofluorescin diacetate (H₂DCF-DA) method. The fourier-transform infrared spectroscopy (FTIR) and Q Exactive plus orbitrap™ Ultra-high-performance liquid chromatography-mass spectrometer (UHPLC-MS) analysis was conducted to evaluate phytochemical constituents of the extracts. The plant extracts selected in this study displayed potency against the Candida species tested, with MIC values ≤0.62 mg/mL for V. karroo, V. kosiensis and V. xanthophloea. A dose-dependent cell viability was observed on Vero cells with all extracts showing LC₅₀ values >20 μg/mL. Extracts tested at 10 μg/mL elicited a significant decrease in lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in Vero cells with V. sieberiana, V. tortilis, V. karroo, V. kosiensis and V. xanthophloea displaying inhibitory percentages of 35%, 32%, 55%, 52% and 49%, respectively. Characterisation of functional groups representing compounds in the extracts demonstrated the presence of different classes of compounds of the aliphatic, sugar and aromatic types. The Q Exactive plus orbitrap™ mass spectrometer enabled tentative identification of three major compounds in the extracts, including epigallocatechin, methyl gallate and quercetin amongst others. Based on the mass spectrometer results, it is postulated that quercetin found mostly in active extracts of V. karroo, V. xanthophloea, and V. kosiensis may be responsible for the observed antifungal and anti-inflammatory activity. This data demonstrates that the Vachellia species that were investigated could potentially be promising candidates for the management of fungal infections and related inflammation.

Keywords: Anti-inflammatory; Anticandidal activity; FTIR; H2DCF-DA; Phytochemical; ROS; UHPLC–Q/Orbitrap/MS; Vachellia.