In vitro and in vivo evaluation of probiotic properties of Corynebacterium accolens isolated from the human nasal cavity

Martha Alemayehu Menberu; Clare Cooksley; Mahnaz Ramezanpour; George Bouras; Peter-John Wormald; Alkis James Psaltis; Sarah Vreugde

doi:10.1016/j.micres.2021.126927

In vitro and in vivo evaluation of probiotic properties of Corynebacterium accolens isolated from the human nasal cavity

Microbiol Res. 2021 Nov 23:255:126927. doi: 10.1016/j.micres.2021.126927. Online ahead of print.

Authors

Martha Alemayehu Menberu¹, Clare Cooksley², Mahnaz Ramezanpour², George Bouras², Peter-John Wormald², Alkis James Psaltis², Sarah Vreugde³

Affiliations

¹ Department of Surgery-Otolaryngology, Head and Neck Surgery, The University of Adelaide, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville, SA, Australia; Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Ethiopia.
² Department of Surgery-Otolaryngology, Head and Neck Surgery, The University of Adelaide, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville, SA, Australia.
³ Department of Surgery-Otolaryngology, Head and Neck Surgery, The University of Adelaide, Basil Hetzel Institute for Translational Health Research, Central Adelaide Local Health Network, Woodville, SA, Australia. Electronic address: sarah.vreugde@adelaide.edu.au.

PMID: 34875424
DOI: 10.1016/j.micres.2021.126927

Abstract

Corynebacterium accolens strains are increasingly recognized as beneficial bacteria that can confer a health benefit on the host. In the current study, the probiotic potential of three C. accolens strains, C779, C781 and C787 derived from a healthy human nasal cavity were investigated. These strains were examined for their adhesion to HNECs, competition with Staphylococcus aureus for adhesion, toxicity, induction of IL-6, antibiotic susceptibility and the presence of antibiotic resistance and virulence genes. Furthermore, the safety and efficacy of strains were evaluated in vivo using Caenorhabditis elegans. The adhesion capacity of C. accolens to HNECs was strain-dependent. Highest adhesion was observed for strain C781. None of the C. accolens strains tested caused cell lysis. All strains were able to outcompete S. aureus for cell adhesion and caused a significant decrease of IL-6 production by HNECs co-exposed to S. aureus when compared to the control groups. All strains were sensitive or showed intermediate sensitivity to 10 different antibiotics. Whole Genome Sequence analysis showed C. accolens C781 and C787 did not possess antibiotic resistance genes whereas strain C779 harboured 5 genes associated with resistance to Aminoglycoside, Chloramphenicol and Erythromycin. In addition, no virulence genes were detected in any of the 3 strains. Moreover, the tested strains had no detrimental effect on worm survival and induced protection from S. aureus-mediated infection. Taken all together, C. accolens strains, C781 and C787 displayed probiotic potential and hold promise for use in clinical applications for combating dysbiosis in chronic rhinosinusitis.

Keywords: Chronic rhinosinusitis; Corynebacteriumaccolens; Dysbiosis; Human nasal cavity; Probiotic.