Human milk-based fortifier is associated with less alteration of milk fat globule size than cow milk-based fortifier

Yurika Yoshida; Minami Azuma; Haruhiro Kuwabara; Tokuo Miyazawa; Yuya Nakano; Kazuna Furukawa; Keli M Hawthorne; Masahiko Izumizaki; Takashi Takaki; Mari Sakaue; Katsumi Mizuno

doi:10.1371/journal.pone.0257491

Human milk-based fortifier is associated with less alteration of milk fat globule size than cow milk-based fortifier

PLoS One. 2021 Dec 7;16(12):e0257491. doi: 10.1371/journal.pone.0257491. eCollection 2021.

Authors

Affiliations

¹ Department of Pediatrics, Showa University School of Medicine, Tokyo, Japan.
² Department of Pediatrics, Dell Medical School, Dell Pediatric Research Institute, University of Texas at Austin, Austin, Texas.
³ Department of Physiology, Showa University School of Medicine, Tokyo, Japan.
⁴ Division of Electron Microscopy, Showa University School of Medicine, Tokyo, Japan.
⁵ Analysis Systems Solution Development Department, Hitachi High-Tech Corporation, Tokyo, Japan.
⁶ Japanese Human Milk Bank Association, Tokyo, Japan.

Abstract

We aimed to evaluate if human milk-based fortifier (HMBF) affects human milk fat globule (MFG) size less than cow milk-based fortifier (CMBF), which may impact overall infant feeding tolerance. Measurements of donated human milk were performed before fortification as well as at 1 hour, 24 hours, and 48 hours after fortification with CMBF or HMBF. MFG size in each sample of fortified milk was measured by laser light scattering. MFG size in the fortified milks increased gradually over time. At 24 and 48 hours after fortification, MFG size in the milk with CMBF was larger than that in the milk with HMBF (4.8 ± 0.5 vs 4.3 ± 0.3 μm, p<0.01, 5.1 ± 0.7 vs 4.5 ± 0.4 μm, p = 0.03, respectively). HMBF is associated with less alteration of MFG size than CMBF. This may have an impact on feeding tolerance of very preterm infants.

MeSH terms

Animals
Cattle
Female
Glycolipids / chemistry*
Glycoproteins / chemistry*
Humans
Infant, Extremely Premature
Infant, Newborn
Lipid Droplets / chemistry*
Milk, Human / chemistry*

Substances

Glycolipids
Glycoproteins
milk fat globule

Grants and funding

KH receives speaker honoraria from Prolacta Bioscience. Prolacta Bioscience (www.prolacta.com) provided the product for use in this study but was not involved in the study design, data collection or analysis, performance of the study, outcomes, decision to publish, or manuscript writing.