Tissue-specific distribution of primary and secondary metabolites of Baemoochae (×Brassicoraphanus) and its changes as a function of developmental stages

Abstract

The distribution and changes in the primary and secondary metabolite profiles of Baemoochae, an inter-generic hybrid of Chinese cabbage and radish, during the plant's developmental stages were investigated. Metabolites were analyzed using gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-electrospray ionization-quadrupole time-of-flight (UHPLC-ESI-qTOF MS). Free sugar, organic acid, and amino acid composition depended on the tissue type and developmental stage of Baemoochae. For example, glucose and alanine levels were higher in mature leaves than in young leaves; citric acid content in mature roots was lower than that in young roots. Several glucosinolates were identified for the first time in Baemoochae. Glucoraphasatin was predominant in both leaves and roots, regardless of plant maturity. Total glucosinolate content was significantly higher in roots than in leaves and in mature than in young plants. The roots of mature Baemoochae could be used as a rich source of glucosinolates, with several potential health-promoting effects.

Keywords: Brassica; Developmental stage; Glucoraphasatin; Glucosinolate; Metabolite profiling.