Type-III interferon stimulated gene TRIM31 mutation in an HBV patient blocks its ability in promoting HBx degradation

Fengchao Xu; Hongxiao Song; Qingfei Xiao; Qi Wei; Xiaoli Pang; Yanli Gao; Guangyun Tan

doi:10.1016/j.virusres.2021.198650

Type-III interferon stimulated gene TRIM31 mutation in an HBV patient blocks its ability in promoting HBx degradation

Virus Res. 2022 Jan 15:308:198650. doi: 10.1016/j.virusres.2021.198650. Epub 2021 Dec 1.

Authors

Fengchao Xu¹, Hongxiao Song¹, Qingfei Xiao², Qi Wei³, Xiaoli Pang⁴, Yanli Gao⁵, Guangyun Tan⁶

Affiliations

¹ Department of Immunology, Institute of Translational Medicine, the First Hospital of Jilin University, Changchun, Jilin 130061, China.
² Department of Nephrology, the First Hospital of Jilin University, Changchun, 130031, Jilin, China.
³ Department of Anesthesia, the First Hospital, Jilin University, Changchun, 130021, Jilin, China.
⁴ Department of Pediatric Gastroenterology, the First Hospital, Jilin University, Changchun 130021, Jilin, China.
⁵ Department of Pediatrics, the First Hospital, Jilin University, Changchun 130021, Jilin, China. Electronic address: gaoyanli@jlu.edu.cn.
⁶ Department of Immunology, Institute of Translational Medicine, the First Hospital of Jilin University, Changchun, Jilin 130061, China. Electronic address: tgy0425@jlu.edu.cn.

PMID: 34863820
DOI: 10.1016/j.virusres.2021.198650

Abstract

TRIM5γ, together with TRIM31, has been shown to promote HBx ubiquitination and degradation. This study aimed to explore whether a patient with HCC (hepatic cell carcinoma) having a small nucleotide inserted into the TRIM31 gene, which made a shorter transcript stop at 768 bp, would result in blocking the activity of TRIM31 in promoting HBx degradation. Besides, this study aimed to determine the binding region of the TRIM31-TRIM5γ-HBx complex. HBV (Hepatitis B virus) infection was reported to induce type-III IFN but not type-I or type-II IFNs, here TRIM31 was found to be a type III rather than a type I stimulated gene, which was indispensable in inhibiting the hepatitis B virus replication by the interferon families. Thus, this study further identified the critical role of TRIM31 in the host-hepatitis B virus interaction.

Keywords: HBx; Hepatitis B virus (HBV); Interferon; TRIM31.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Hepatocellular* / genetics
Hep G2 Cells
Hepatitis B virus / genetics
Hepatitis B virus / metabolism
Hepatitis B*
Humans
Interferons / genetics
Liver Neoplasms*
Mutation
Trans-Activators / genetics
Trans-Activators / metabolism*
Tripartite Motif Proteins / genetics
Ubiquitin-Protein Ligases / genetics
Ubiquitin-Protein Ligases / metabolism
Viral Regulatory and Accessory Proteins / genetics
Viral Regulatory and Accessory Proteins / metabolism*
Virus Replication / genetics

Substances

Trans-Activators
Tripartite Motif Proteins
Viral Regulatory and Accessory Proteins
hepatitis B virus X protein
Interferons
TRIM31 protein, human
Ubiquitin-Protein Ligases