Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system

Seung-Hun Kim; Kwang-Hwan Choi; Jinsol Jeong; Mingyun Lee; Dong-Kyung Lee; Jong-Nam Oh; Gyeong Cheol Choe; Du-Min Go; Dae-Yong Kim; Chang-Kyu Lee

doi:10.1016/j.scr.2021.102609

Pig embryonic stem cell line with porcine-specific OCT4 upstream region based dual reporter system

Stem Cell Res. 2021 Dec:57:102609. doi: 10.1016/j.scr.2021.102609. Epub 2021 Nov 27.

Authors

Affiliations

¹ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea.
² Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea.
³ Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, Republic of Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea. Electronic address: leeck@snu.ac.kr.

PMID: 34861590
DOI: 10.1016/j.scr.2021.102609

Abstract

Here, we report pig embryonic stem cells (ESCs) originating from parthenogenetic blastocysts which were developed from 2-cell embryos micro-injected with porcine OCT4 reporter system cultured in previous reported chemically defined culture media. The ESCs with reporter system expressed pluripotency markers and fluorescent signals produced by OCT4 reporter system. Also, they were capable of forming teratomas following subcutaneous injection into nude mice. Since reporter system enables the non-destructive classification of the condition of live pluripotent stem cells, this reporter cell line could be a useful resource for research on species-specific pluripotency.