The allelopathy and underlying mechanism of Skeletonema costatum on Karenia mikimotoi integrating transcriptomics profiling

Xin Guo; Tongzhu Han; Liju Tan; Ting Zhao; Xiaolin Zhu; Wenqiu Huang; Kun Lin; Na Zhang; Jiangtao Wang

doi:10.1016/j.aquatox.2021.106042

The allelopathy and underlying mechanism of Skeletonema costatum on Karenia mikimotoi integrating transcriptomics profiling

Aquat Toxicol. 2022 Jan:242:106042. doi: 10.1016/j.aquatox.2021.106042. Epub 2021 Nov 22.

Authors

Xin Guo¹, Tongzhu Han¹, Liju Tan¹, Ting Zhao², Xiaolin Zhu¹, Wenqiu Huang¹, Kun Lin¹, Na Zhang¹, Jiangtao Wang³

Affiliations

¹ Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China.
² Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China; Eco-Environmental Monitoring and Research Center, Pearl River Valley and South China Sea Ecology and Environment Administration, Ministry of Ecology and Environment, PCR, Guangzhou, 510610, China.
³ Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China. Electronic address: jtwang@ouc.edu.cn.

PMID: 34861574
DOI: 10.1016/j.aquatox.2021.106042

Abstract

The roles of allelopathy for succession of marine phytoplankton communities remain controversial, especially for the development of blooms. Physiological parameters measurement (Fv/Fm value, MDA content, SOD activity, Na⁺/K⁺, Ca²⁺/ Mg²⁺-ATPase activity, cell size, chlorophyll content, apoptosis and cell cycle) and whole transcriptome profiling analysis were used to investigate allelopathy effect of Skeletonema costatum on Karenia mikimotoi. Filtrate and extracts from S. costatum culture inhibited the growth of K. mikimotoi. Allelopathic effects were dose-dependent for filtrate culture and extract culture. K. mikimotoi scavenged excessive ROS and adapted to the stress fastly and easily, so oxidative damage was not the main cause of the growth inhibition. Allelochemicals of S. costatum were found to influence the structure and function of cell membrane of K. mikimotoi by damaging membrane structure till to cell necrosis, which caused high mortality. Coupled with the sensitivity of algal cells to environmental stress and restricted cell cycle, allelopathy was suggested to be deeply detrimental to the development of competition algal population.

Keywords: Allelochemicals; Karenia mikimotoi; Oxidative damage; RNA-seq; Skeletonema costatum.

MeSH terms

Allelopathy*
Diatoms*
Dinoflagellida*
Gene Expression Profiling
Transcriptome