Complete Genome Sequence of the Virulent Klebsiella pneumoniae Phage Geezett Infecting Multidrug-Resistant Clinical Strains

Belinda Loh; Liwei Zhang; Xiaoting Hua; Yunsong Yu; Long Ma; Xiaoqing Wang; Prasanth Manohar; Ramesh Nachimuthu; Willames M B S Martins; Mark A Toleman; Sebastian Leptihn

doi:10.1128/MRA.00685-21

Complete Genome Sequence of the Virulent Klebsiella pneumoniae Phage Geezett Infecting Multidrug-Resistant Clinical Strains

Microbiol Resour Announc. 2021 Dec 2;10(48):e0068521. doi: 10.1128/MRA.00685-21. Epub 2021 Dec 2.

Authors

Belinda Loh¹, Liwei Zhang², Xiaoting Hua³, Yunsong Yu³, Long Ma², Xiaoqing Wang^{2

4}, Prasanth Manohar², Ramesh Nachimuthu⁵, Willames M B S Martins⁶, Mark A Toleman⁶, Sebastian Leptihn^{2

3

7}

Affiliations

¹ Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, Suzhou, China.
² Zhejiang University-University of Edinburgh (ZJU-UoE) Institute, Zhejiang University, International Campus, Haining, Zhejiang, China.
³ Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
⁴ Medical School, Lishui University, Lishui, China.
⁵ Antibiotic Resistance and Phage Therapy Laboratory, School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu, India.
⁶ Department of Medical Microbiology, Division of Infection and Immunity, Cardiff University, Cardiff, United Kingdom.
⁷ College of Medicine & Veterinary Medicine, University of Edinburgh Medical School, Edinburgh, United Kingdom.

Abstract

Geezett was isolated from hospital sewage in Hangzhou, China, and exhibits lytic activity against clinical isolates of the nosocomial pathogen Klebsiella pneumoniae. The bacteriophage is a myovirus and has a double-stranded DNA (dsDNA) genome 50,707 bp long, containing 79 open reading frames (ORFs).

Grants and funding

32011530116/National Natural Science Foundation of China (NSFC)