Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

Mitchell DiPasquale; Michael H L Nguyen; Stuart R Castillo; Frederick A Heberle; Drew Marquardt

doi:10.1007/978-1-0716-1843-1_14

Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering

Methods Mol Biol. 2022:2402:163-177. doi: 10.1007/978-1-0716-1843-1_14.

Authors

Mitchell DiPasquale¹, Michael H L Nguyen¹, Stuart R Castillo¹, Frederick A Heberle², Drew Marquardt^{3

4}

Affiliations

¹ Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.
² Department of Chemistry, University of Tennessee, Knoxville, TN, USA.
³ Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada. drew.marquardt@uwindsor.ca.
⁴ Department of Physics, University of Windsor, Windsor, ON, Canada. drew.marquardt@uwindsor.ca.

PMID: 34854044
DOI: 10.1007/978-1-0716-1843-1_14

Abstract

Lipid domains in model membranes are routinely studied to provide insight into the physical interactions that drive raft formation in cellular membranes. Using small angle neutron scattering, contrast-matching techniques enable the detection of lipid domains ranging from tens to hundreds of nanometers which are not accessible to other techniques without the use of extrinsic probes. Here, we describe a probe-free experimental approach and model-free analysis to identify lipid domains in freely floating vesicles of ternary phase separating lipid mixtures.

Keywords: Contrast-matching; Domains; Lipid rafts; Lipid vesicles; Phase separation; Small angle neutron scattering.

MeSH terms

Biophysical Phenomena
Lipid Bilayers
Lipids*
Membrane Microdomains
Neutron Diffraction
Neutrons
Scattering, Small Angle*

Substances

Lipid Bilayers
Lipids