Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

Lissy M Hartmann; Alvaro Garcia; Evelyne Deplazes; Charles G Cranfield

doi:10.1007/978-1-0716-1843-1_7

Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes

Methods Mol Biol. 2022:2402:81-92. doi: 10.1007/978-1-0716-1843-1_7.

Authors

Lissy M Hartmann¹, Alvaro Garcia², Evelyne Deplazes², Charles G Cranfield²

Affiliations

¹ School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia. lissy.hartmann@uts.edu.au.
² School of Life Sciences, University of Technology Sydney, Ultimo, NSW, Australia.

PMID: 34854037
DOI: 10.1007/978-1-0716-1843-1_7

Abstract

Swept frequency electrical impedance spectroscopy (EIS) can be used in conjunction with tethered bilayer lipid membranes to monitor the membrane permeability of ions in real-time (Deplazes et al. J Phys Chem Lett 11:6353-6358, 2020). Conductance readings, as determined by EIS, are a measure of the ability of ions to be transported across membranes. Recording the change in conductance as a function of cation concentration and a comparison between a range of cations permits conclusions to be made about the specificity of cation transport through pores. An estimate for upper pore size and cation selectivity of ion channels can be established using this method.

Keywords: Cation transport; Electrical impedance spectroscopy; Ion channels; Tethered bilayer lipid membranes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cations
Dielectric Spectroscopy
Ion Channels
Lipid Bilayers*
Peptides

Substances

Cations
Ion Channels
Lipid Bilayers
Peptides