Triiodothyronine Aggravates Global Cerebral Ischemia-Reperfusion Injury in Mice

Biol Pharm Bull. 2021;44(12):1824-1831. doi: 10.1248/bpb.b21-00424.

Abstract

Thyroid hormones (THs) have been suggested to play an important role in both physiological and pathological events in the central nervous system. Hypothyroidism, which is characterized by low levels of serum THs, has been associated with aggravation of ischemic neuronal injuries in stroke patients. We hypothesized that administration of T3, the main active form of THs, may attenuate the ischemic neuronal injuries. In mice, global cerebral ischemia (GCI), which is induced by transient occlusion of the bilateral common carotid artery, causes neuronal injuries by inducing neuronal death and activating inflammatory responses after reperfusion in the hippocampus. In this study, we examined the effect of T3 administration on DNA fragmentation induced by neuronal death and the activation of inflammatory cells such as astrocytes and microglia in the hippocampus following GCI. The content of nucleosomes generated by DNA fragmentation in the hippocampus was increased by GCI and further increased by T3 administration. The protein expression levels of glial fibrillary acidic protein (GFAP), an astrocytic marker, and Ionized calcium binding adaptor protein 1 (Iba1), a microglial marker, in the hippocampus were also increased by GCI and further increased by T3 administration. The levels of T3 in both the serum and hippocampus were elevated by T3 administration. Our results indicate that T3 administration aggravates GCI-reperfusion injury in mice. There may be an increased risk of aggravation of ischemic stroke by the excessive elevation of T3 levels during the drug treatment of hypothyroidism.

Keywords: global cerebral ischemia; neuronal injury; triiodothyronine.

MeSH terms

  • Animals
  • Astrocytes
  • Brain Ischemia*
  • Cell Death
  • Cerebral Infarction
  • DNA Fragmentation
  • Disease Models, Animal
  • Glial Fibrillary Acidic Protein
  • Hippocampus / drug effects*
  • Hypothyroidism / complications
  • Hypothyroidism / drug therapy
  • Inflammation
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microglia
  • Neurons
  • Nucleosomes
  • Reperfusion
  • Reperfusion Injury*
  • Severity of Illness Index*
  • Triiodothyronine / adverse effects*
  • Triiodothyronine / blood

Substances

  • Glial Fibrillary Acidic Protein
  • Nucleosomes
  • glial fibrillary astrocytic protein, mouse
  • Triiodothyronine