This paper aims to identify the organisational culture profiles of Polish municipalities and examine the influence of the professional, social and political experience and place-based leadership style of mayors on municipalities' organisational culture profiles. The Organisational Culture Assessment Instrument was selected due to its suitability in assessing the organisation's underlying culture. In the study, 917 mayors of municipalities in Poland, completing an on-line questionnaire. It was found that most of the Polish municipalities' organisational culture is characterised by a clan type which is reflected in how employees are managed, how the organisation is held together, and how the organisation's strategy is defined. The leadership style and the organisation's success are hierarchy-focused, while the dominant characteristic is market type. The ANOVA and UNIANOVA analysis results suggest that the type of organisational culture depends on the type of municipality. The clan culture is dominant in rural municipalities. In urban municipalities, market culture and adhocracy are stronger than in rural municipalities, while clan culture is weaker. There is also an association between the dominant type of organisational culture and the mayors' work experience in local administration and their membership in an NGO. The length of the mayor's seniority in local government administration differentiates the importance of hierarchy culture, while his experience in the NGO sector strengthens the clan characteristics of the organisational culture of the office he heads. These findings provide important implications for the initiation and implementation of cultural change in local government administration and cooperation projects and local experiments. A cultural change is difficult to implement, and a change of mayor is not enough to initiate it. It requires planning and management. Cultural change may contribute to the increase of municipalities' activity in cooperation's projects and experiments. There is a need for more research on this topic to determine to what extent the organisational culture supports local cooperation projects.