Laboratory virulence of entomopathogenic nematodes to the sweetpotato whitefly, Bemisia tabaci

Yinping Li; George N Mbata; David I Shapiro-Ilan

doi:10.21307/jofnem-2021-096

Laboratory virulence of entomopathogenic nematodes to the sweetpotato whitefly, Bemisia tabaci

J Nematol. 2021 Nov 26:53:e2021-96. doi: 10.21307/jofnem-2021-096. eCollection 2021.

Authors

Yinping Li¹, George N Mbata¹, David I Shapiro-Ilan²

Affiliations

¹ Agricultural Research Station, Fort Valley State University, 1005 State University Drive, Fort Valley, GA 31030.
² USDA, Agricultural Research Service, Southeastern Fruit and Tree Nut Research Laboratory, 21 Dunbar Road, Byron, GA 31008.

Abstract

The sweetpotato whitefly, Bemisia tabaci Middle East-Asia Minor 1 (MEAM1), is a major insect pest on vegetable crops worldwide. Enormous economic losses result from direct and indirect plant damage caused by MEAM1. Biological control using entomopathogenic nematodes (EPN) may be an effective alternative strategy against MEAM1 because this pest has developed resistance to most insecticides. First, nine EPN species (Heterorhabditis bacteriophora, H. indica, H. georgiana, H. floridensis, Steinernema feltiae, S. carpocapsae, S. riobrave, S. glaseri, and S. rarum) were investigated for virulence to MEAM1 third instar nymphs on snap bean leaves under laboratory conditions. The mortality of MEAM1 nymphs was evaluated at 3 days post-inoculation (dpi). Compared to the water control, the application of the nine EPN species except S. glaseri resulted in significantly higher mortality of MEAM1 nymphs, such as H. bacteriophora (66.31%), H. floridensis (56.38%), S. carpocapsae (54.54%), and S. rarum (57.80%). Subsequently, the four virulent EPN species, H. bacteriophora, H. floridensis, S. carpocapsae, and S. rarum were evaluated further for virulence to MEAM1 nymphs on snap bean and tomato leaves. The mortality of MEAM1 nymphs was assessed at 3 dpi and 7 dpi. There were no significant differences in MEAM1 nymphal mortality between tomato and snap bean at either 3 dpi or 7 dpi. The mortality of MEAM1 nymphs caused by the application of H. floridensis (99.25%) was significantly higher than the other three EPN species and the water control at 7dpi. The results indicate that H. floridensis is a very promising biocontrol agent for B. tabaci management.

Keywords: Bemisia tabaci; Biological control; Entomopathogenic nematode; Laboratory virulence; Plant protection; Snap bean; Sweetpotato whitefly; Tomato.