Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs

Andrey Anisenko; Olga Shadrina; Irina Garanina; Marina Gottikh

doi:10.1016/j.dib.2021.107596

Transcriptome dataset of HEK293T cells depleted of one of the subunits of the DNA-PK complex: Ku70, Ku80 or DNA-PKcs

Data Brief. 2021 Nov 19:39:107596. doi: 10.1016/j.dib.2021.107596. eCollection 2021 Dec.

Authors

Andrey Anisenko^{1

2

3}, Olga Shadrina^{1

2}, Irina Garanina⁴, Marina Gottikh^{1

2}

Affiliations

¹ Chemistry Department, Lomonosov Moscow State University, Moscow 199991, Russia.
² Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
³ Department of Boiengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 199991, Russia.
⁴ Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, Russia.

Abstract

DNA-PK is a heterotrimeric complex that consists of Ku70 (XRCC6), Ku80 (XRCC5) and DNA-PKcs (PRKDC) subunits. The complex is a major player in the repair of DNA double strand break (DSB) via the non-homologous end joining (NHEJ) pathway. This process requires all DNA-PK subunits, since Ku70/Ku80 heterodimer firstly binds to DNA ends at DSB and then recruits DNA-PKcs. Recruitment of the DNA-PKcs subunit to DSB leads to phosphorylation events near DSB and recruitment of other NHEJ-related proteins that restore DNA integrity. However, today a lot of evidence demonstrates participation of the DNA-PK components in other cellular processes, e.g. telomere length maintenance, transcription, metabolism regulation, cytosolic DNA sensing, apoptosis, cellular movement and adhesion. It is important to note that not all the subunits of the DNA-PK complex are necessary for these processes, and the largest number of independent functions has been shown for the Ku70/Ku80 heterodimer and especially the Ku70 subunit. To better understand the role of each DNA-PK subunit in the cell life, we have analyzed transcriptome changes in HEK293T cells depleted of Ku70, Ku80 or DNA-PKcs using NGS-sequencing. Here, for the first time, we present the data obtained from the transcriptome analysis.

Keywords: DNA-PKcs; DNA-dependent protein kinase; Ku heterodimer; Ku70; Ku80; NHEJ-related proteins.