Integrated Analysis of the m6A-Related lncRNA Identified lncRNA ABALON/miR-139-3p/ NOB1 Axis Was Involved in the Occurrence of Lung Cancer

Xinhong Liu; Xin Zuo; Lijun Ma; Qin Wang; Lilan Zhu; Li Li; Xin Zhao

doi:10.2147/CMAR.S339032

Integrated Analysis of the m6A-Related lncRNA Identified lncRNA ABALON/miR-139-3p/ NOB1 Axis Was Involved in the Occurrence of Lung Cancer

Cancer Manag Res. 2021 Nov 23:13:8707-8722. doi: 10.2147/CMAR.S339032. eCollection 2021.

Authors

Xinhong Liu^#^{1

2

3

4}, Xin Zuo^#⁵, Lijun Ma^{1

2

3

4}, Qin Wang^{1

2

3

4}, Lilan Zhu^{1

2

3

4}, Li Li^{1

2

3

4}, Xin Zhao^{2

3

4}

Affiliations

¹ College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, People's Republic of China.
² Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, People's Republic of China.
³ Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, People's Republic of China.
⁴ Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, People's Republic of China.
⁵ Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, People's Republic of China.

^# Contributed equally.

Abstract

Background: Lung cancer has the characteristics of early metastasis, high recurrence, and high mortality rate despite emerging advances in diagnostic. Early diagnosis can significantly improve the patient's chances of cure and survival.

Purpose: This study aimed to identify and assess a prognostic lncRNA/miRNA/gene signature in patients with lung cancer.

Methods: Pearson correlation analysis, univariate Cox analysis and LASSO Cox analysis were used to construct a lung cancer prognostic risk model based on m6A-related lncRNA. The interaction between lncRNA-miRNA-gene was verified by luciferase reporter gene experiment.

Results: The Pearson correlation analysis determined that 1655 lncRNAs significantly correlated with the expression of m6A genes. A lung cancer prognostic risk model, including 14 m6A-related lncRNAs, was constructed through univariate Cox analysis and least absolute shrinkage and selection operator (LASSO) Cox analysis. ABALON was identified as the key lncRNA through cluster analysis and gene expression difference analysis.

Conclusion: It was experimentally verified that ABALON acted as a competing endogenous RNA by sponging miR-139-3p and indirectly regulated the expression of NOB1. This study provided a new biological target for the early diagnosis of lung cancer and a new direction for studying the mechanism of lung cancer.

Keywords: N6-methyladenosine; lncRNA; lung cancer; miRNA; prognostic biomarker.

Grants and funding

This research was funded by Chongqing University Innovation Research Group Project (CXQTP20033), the Science and Technology Project of Chongqing Education Commission (KJQN202001604), and Natural Science Foundation of Chongqing Science and Technology Commission (cstc2020jcyj-msxmX0874), China.