Collective bacterial disinfection by opto-chemical treatment on mature biofilm in clinical endoscope

Van Nam Tran; Suhyun Park; Fazlurrahman Khan; Van Gia Truong; Seok Jeong; Don Haeng Lee; Young-Mog Kim; Hyun Wook Kang

doi:10.1016/j.jphotobiol.2021.112367

Collective bacterial disinfection by opto-chemical treatment on mature biofilm in clinical endoscope

J Photochem Photobiol B. 2022 Jan:226:112367. doi: 10.1016/j.jphotobiol.2021.112367. Epub 2021 Nov 22.

Authors

Van Nam Tran¹, Suhyun Park², Fazlurrahman Khan³, Van Gia Truong¹, Seok Jeong⁴, Don Haeng Lee⁴, Young-Mog Kim⁵, Hyun Wook Kang⁶

Affiliations

¹ Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, South Korea.
² Department of Electronic and Electrical Engineering, Ewha Womans University, Seoul 03760, South Korea.
³ Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, South Korea.
⁴ Division of Gastroenterology, Department of Internal Medicine, Inha University College of Medicine, Incheon 22212, South Korea.
⁵ Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, South Korea; Department of Food Science and Technology, Pukyong National University, Busan 48513, South Korea.
⁶ Industry 4.0 Convergence Bionics Engineering, Pukyong National University, Busan 48513, South Korea; Research Center for Marine Integrated Bionics Technology, Pukyong National University, Busan 48513, South Korea; Department of Biomedical Engineering, Pukyong National University, Busan 48513, South Korea. Electronic address: wkang@pukyong.ac.kr.

PMID: 34847498
DOI: 10.1016/j.jphotobiol.2021.112367

Abstract

The present study proposes an innovative opto-chemical treatment using a basket-integrated optical device (BIOD) to disinfect mature bacterial biofilm on endoscope channels. A BIOD was designed to position an optical diffuser on the central axis of an endoscope channel and to distribute laser light concentrically to the bacterial biofilm on the channel surface. To apply thermal damage and oxidative stress to the bacterial biofilm, a low concentration of a crosslinking agent (glutaraldehyde ~0.5%) was combined with 808 nm infrared (IR) and 405 nm blue (BL) laser lights. The applied irradiances of IR and BL were 10 W/cm² and 1.6 W/cm² for Teflon channel model and 20 W/cm² and 3.2 W/cm² for a clinical model, respectively. Individual irradiation of either IR or BL for 180 s induced the maximum temperatures of 62 ± 2 °C and 53 ± 3 °C on the biofilm, respectively. The simultaneous opto-chemical treatment reduced a significant population of the bacterial biofilms (7.5-log₁₀ for Staphylococcus aureus and 7.1-log₁₀ for Pseudomonas aeruginosa), which were 2.9-fold and 3.9-fold higher than that of the standard treatment with 2% glutaraldehyde (GA) solution, respectively. The proposed opto-chemical disinfection method can help reduce multi-drug resistant bacteria and prevent cross-infection during the clinical usage of a flexible endoscope.

Keywords: Bacterial biofilm; Blue light; Disinfection; Flexible endoscope; Glutaraldehyde; Infrared light.

MeSH terms

Biofilms*