Detection of Viroids by RT-PCR

Nerida J Donovan; Grant A Chambers; Mengji Cao

doi:10.1007/978-1-0716-1464-8_13

Detection of Viroids by RT-PCR

Methods Mol Biol. 2022:2316:143-151. doi: 10.1007/978-1-0716-1464-8_13.

Authors

Nerida J Donovan¹, Grant A Chambers², Mengji Cao³

Affiliations

¹ Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia. nerida.donovan@dpi.nsw.gov.au.
² Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
³ National Citrus Engineering Research Centre, Citrus Research Institute, Southwest University, Chongqing, China.

PMID: 34845692
DOI: 10.1007/978-1-0716-1464-8_13

Abstract

Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels. Methods of viroid detection using conventional RT-PCR are described in this chapter.

Keywords: Gel electrophoresis; Primers; Reverse transcription-polymerase chain reaction (RT-PCR); Viroid detection.

MeSH terms

DNA Primers
DNA, Complementary
Plant Diseases
Plants
Reverse Transcriptase Polymerase Chain Reaction
Viroids* / genetics

Substances

DNA Primers
DNA, Complementary