Physiological response of soybean leaves to uniconazole under waterlogging stress at R1 stage

Shiya Wang; Hang Zhou; Naijie Feng; Hongtao Xiang; Yang Liu; Feng Wang; Wan Li; Shengjie Feng; Meiling Liu; Dianfeng Zheng

doi:10.1016/j.jplph.2021.153579

Physiological response of soybean leaves to uniconazole under waterlogging stress at R1 stage

J Plant Physiol. 2022 Jan:268:153579. doi: 10.1016/j.jplph.2021.153579. Epub 2021 Nov 24.

Authors

Shiya Wang¹, Hang Zhou², Naijie Feng³, Hongtao Xiang⁴, Yang Liu⁵, Feng Wang⁶, Wan Li⁴, Shengjie Feng², Meiling Liu², Dianfeng Zheng⁷

Affiliations

¹ College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China; College of Agriculture, Heilongjiang Bayi Agriculture University, Daqing, 163319, China.
² College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
³ College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China; Shenzhen Research Institute, Guangdong Ocean University, Shenzhen, 518108, China.
⁴ Institute of Crop Cultivation and Tillage, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, China.
⁵ Yantai Academy of Agricultural Sciences, Shandong province, Yantai, 265500, China.
⁶ Qiqihar Agricultural Technology Extension Center, Qiqihar, 161006, China.
⁷ College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China; Shenzhen Research Institute, Guangdong Ocean University, Shenzhen, 518108, China. Electronic address: zhengdf@gdou.edu.cn.

PMID: 34839099
DOI: 10.1016/j.jplph.2021.153579

Abstract

Waterlogging is a major limiting factor in global crop production and seriously endangers growth and yield improvement in low-lying, rainfed regions. Soybean is an important economic crop affected by waterlogging stress. The current study investigates the effects of waterlogging stress on the leaf physiology and yield of two soybean varieties (Kenfeng 14, waterlogging-tolerant and Kenfeng 16, waterlogging-sensitive) and the mitigation effect of uniconazole (S3307) in promoting growth and productivity under waterlogging conditions. The results showed that waterlogging stress increased antioxidant enzyme activity and decreased the contents of non-enzymatic antioxidants such as AsA and GSH. Furthermore, the content of MDA and H₂O₂ increased significantly, indicating oxidative stress and O₂^-· production rate also improved, and the increase in the waterlogging-sensitive variety Kenfeng 16 was greater than that of the waterlogging-tolerant variety Kenfeng 14. Spraying S3307, however, increased the activities of antioxidants such as SOD, POD, CAT, and APX. GR, MDHAR, and DHAR increased the content of non-enzymatic antioxidants, effectively inhibited the increase of MDA, H₂O₂ content, and O₂^-· production rate, and alleviated the loss of yield factors caused by waterlogging stress. The waterlogging-tolerant variety Kenfeng 14 recovered better than the waterlogging-sensitive variety Kenfeng 16. In summary, S3307 ameliorated the effects of waterlogging stress on the physiological characteristics of soybean leaves and improved yield as a result of improved antioxidant defense mechanisms that impeded lipid peroxidation. Thus, S3307 could decelerate the damages caused by waterlogging stress to some extent.

Keywords: AsA–GSH cycle; Enzymatic defense system; Soybean; Uniconazole; Yield.

MeSH terms

Antioxidants / metabolism
Floods*
Glycine max* / physiology
Hydrogen Peroxide
Plant Leaves / physiology*
Stress, Physiological*
Triazoles / pharmacology*
Water / physiology

Substances

Antioxidants
Triazoles
Water
Hydrogen Peroxide
uniconazole