Complete Genome Sequences of Klebsiella michiganensis and Citrobacter farmeri, KPC-2-Producers Serially Isolated from a Single Patient

Jehane Y Abed; Maxime Déraspe; Ève Bérubé; Matthew D'Iorio; Ken Dewar; Maurice Boissinot; Jacques Corbeil; Michel G Bergeron; Paul H Roy

doi:10.3390/antibiotics10111408

Complete Genome Sequences of Klebsiella michiganensis and Citrobacter farmeri, KPC-2-Producers Serially Isolated from a Single Patient

Antibiotics (Basel). 2021 Nov 18;10(11):1408. doi: 10.3390/antibiotics10111408.

Authors

Jehane Y Abed^{1

2}, Maxime Déraspe^{1

2}, Ève Bérubé¹, Matthew D'Iorio³, Ken Dewar^{4

5}, Maurice Boissinot¹, Jacques Corbeil^{1

6}, Michel G Bergeron^{1

2}, Paul H Roy^{1

7}

Affiliations

¹ Centre de Recherche en Infectiologie, Centre de Recherche du CHU de Québec, Université Laval, 2705 boul. Laurier, Suite R-0709, Québec, QC G1V 4G2, Canada.
² Département de Microbiologie et Immunologie, Pavillon Vandry, Université Laval, Québec, QC G1V 0A6, Canada.
³ McGill Genome Centre, 740 Avenue Docteur-Penfield, Montréal, QC H3A 0G1, Canada.
⁴ Department of Human Genetics, McGill University, 3640 rue University, Rm 2/38F, Montréal, QC H3A 0C7, Canada.
⁵ McGill Centre for Microbiome Research, 3605 de la Montagne, Montréal, QC H3G 2M1, Canada.
⁶ Département de Médecine Moléculaire, Pavillon Vandry, Université Laval, Québec, QC G1V 0A6, Canada.
⁷ Département de Biochimie, de Microbiologie et de Bio-Informatique, Pavillon Vachon, Université Laval, Québec, QC G1V 0A6, Canada.

Abstract

Carbapenemase-producing Enterobacterales, including KPC-2 producers, have become a major clinical problem. During an outbreak in Quebec City, Canada, KPC-2-producing Klebsiella michiganensis and Citrobacter farmeri were isolated from a patient six weeks apart. We determined their complete genome sequences. Both isolates carried nearly identical IncN2 plasmids with blaKPC-2 on a Tn4401b element. Both strains also carried IncP1 plasmids, but that of C. farmeri did not carry a Beta-lactamase gene, whereas that of K. michiganensis carried a second copy of bla_KPC-2 on Tn4401b. These results suggest recent plasmid transfer between the two species and a recent transposition event.

Keywords: Citrobacter farmeri; KPC-2; Klebsiella michiganensis; carbapenemase; plasmid; transposon.