Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing

Pierfrancesco Pagella; Bernd Stadlinger; Thimios A Mitsiadis

doi:10.1016/j.xpro.2021.100953

Isolation of dental pulp and periodontal cells from human teeth for single-cell RNA sequencing

STAR Protoc. 2021 Nov 17;2(4):100953. doi: 10.1016/j.xpro.2021.100953. eCollection 2021 Dec 17.

Authors

Pierfrancesco Pagella¹, Bernd Stadlinger², Thimios A Mitsiadis¹

Affiliations

¹ Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
² Clinic of Cranio-Maxillofacial and Oral Surgery, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.

Abstract

Teeth and the surrounding periodontal tissues are affected by many pathologies that compromise their integrity and significantly affect life quality. The study of the main dental tissues, the dental pulp and periodontium, is made arduous by their close association with highly mineralized tissues (dentin, cementum, and alveolar bone). Here we describe a protocol to isolate all cells composing human dental pulp and periodontium for single-cell RNA sequencing analysis. For complete details on the use and execution of this protocol, please refer to Pagella et al. (2021).

Keywords: Cell Biology; Cell isolation; Genomics; Health Sciences; Single Cell; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques
Cell Separation / methods*
Cells, Cultured
Dental Pulp / cytology*
Humans
Periodontium / cytology*
Sequence Analysis, RNA / methods*
Single-Cell Analysis / methods*
Tooth / cytology