Sulfur-driven autotrophic denitrification (SADN) is a promising technology for nitrogen removal from wastewater. In this study, different-strength SMFs (0, 5, 20, 50, 70 mT) were evaluated to investigate the potential of external static magnetic field (SMF) for enriching sulfur-oxidizing autotrophic denitrifiers (SOAD). 50-mT and 70-mT SMFs were most suitable to accelerate the growth of SOAD and the elimination of non-SOAD. The relative abundance of Thiobacillus significantly increased (p < 0.01) from 6.26% in control reactor to 36.15% under 50 mT and 52.51% under 70 mT. Under 50 mT, Thiobacillus denitrificans accumulated most rapidly, with the largest population. Furthermore, functional gene forecast by high-throughput and metagenomic sequencing indicated that SMF changed the two-component system, the adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling system, the phosphotransferase system (PTS), as well as N/S-related enzymes to regulate stress-response and promote the growth of SOAD. The findings indicated that SMF accelerated the start-up of SADN.
Keywords: Enrichment of functional bacteria; Magnetic biological effect; Magnetic field; Magnetic induction mechanism; Sulfur-driven autotrophic denitrification.
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