ALDOA maintains NLRP3 inflammasome activation by controlling AMPK activation

Dongsheng Bai; Jiaying Du; Xiumin Bu; Wangjia Cao; Tifan Sun; Jiawei Zhao; Yue Zhao; Na Lu

doi:10.1080/15548627.2021.1997051

ALDOA maintains NLRP3 inflammasome activation by controlling AMPK activation

Autophagy. 2022 Jul;18(7):1673-1693. doi: 10.1080/15548627.2021.1997051. Epub 2021 Nov 25.

Authors

Dongsheng Bai¹, Jiaying Du¹, Xiumin Bu¹, Wangjia Cao¹, Tifan Sun¹, Jiawei Zhao¹, Yue Zhao¹, Na Lu¹

Affiliation

¹ State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, Department of Physiology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, People's Republic of China.

Abstract

ALDOA: aldolase A; AMPK: AMP-activated protein kinase; ATG: autophagy related; ATG5: autophagy related 5; ATP: adenosine triphosphate; BMDMs: bone marrow-derived macrophages; CALCOCO2: calcium binding and coiled-coil domain 2; CASP1: caspase 1; CQ: chloroquine; FOXO3: forkhead box O3; IL1B: interleukin 1 beta; LPS: lipopolysaccharide; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MT: mutant; mtDNA: mitochondrial DNA; MTORC1: mechanistic target of rapamycin kinase complex 1; mtROS: mitochondrial reactive oxygen species; NLRP3: NLR family, pyrin domain containing 3; OPTN: optineurin; PBS: phosphate-buffered saline; PRKN/Parkin: parkin RBR E3 ubiquitin protein ligase; SN: supernatant; SQSTM1/p62: sequestosome 1; STK11/LKB1: serine/threonine kinase 11; TOMM20: translocase of outer mitochondrial membrane 20; ULK1: unc-51 like autophagy activating kinase 1; v-ATPase: vacuolar type H+-ATPase; WT: wild-type.

Keywords: ALDOA; AMPK; LYG-202; NLRP3 inflammasome; mitophagy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

AMP-Activated Protein Kinases
Adenosine Triphosphatases
Autophagy* / physiology
Caspase 1
DNA, Mitochondrial / metabolism
Inflammasomes* / metabolism
Lipopolysaccharides
NLR Family, Pyrin Domain-Containing 3 Protein
Ubiquitin-Protein Ligases / metabolism

Substances

DNA, Mitochondrial
Inflammasomes
Lipopolysaccharides
NLR Family, Pyrin Domain-Containing 3 Protein
Ubiquitin-Protein Ligases
AMP-Activated Protein Kinases
Caspase 1
Adenosine Triphosphatases

Grants and funding

This work was supported by the National Natural Science Foundation of China [81903626]; National Natural Science Foundation of China [81872899]; Natural Science Foundation of Jiangsu Province [BK20180576]; Research Innovation Program for College Graduates of Jiangsu Province [1152100016]; Social Development Project of Jiangsu Provincial Science and Technology Department [BE2018711]; Fundamental Research Funds for the Central Universities [No.2632021ZD03].