Objective: To investigate the effects of long-term moderate-small intensity aerobic exercise on the differential expression of proteome in left ventricular muscle of rats, and to screen the target proteins sensitive to moderate-small intensity aerobic exercise stimulation. This study will enrich the basic theory of exercise and fitness and provide new ideas and experimental basis for the rehabilitation treatment of chronic cardiovascular disease. Methods: Twenty male SD rats were randomly divided into exercise group and control group (n=10). The treadmill training model of long-term moderate-small intensity aerobic exercise was established, and the whole protein samples of left ventricular muscle were extracted and separated by two-dimensional gel electrophoresis (2-DE). The two-dimensional gel electrophoresis map was analyzed by Bio PD quest image analysis software. The protein spots with differential expression more than 5 times or down-regulated over 80% after exercise were identified by tandem time-of-flight mass spectrometry (ULGRAFL-FLEX-TOF/TOF). Results: Compared with the group C, the heart weight index of the group E was increased by 32.0%, and the difference was significant (P＜0.05). Compared with the group C, there were 71 protein spots expression were up-regulated≥2 times or down-regulated≥50% in the group E. 4 protein spots expression were up-regulated≥5 times or down-regulated≥80% were identified by mass spectrometry, 3 proteins and 1 unknown protein were identified. Conclusion: After long-term moderate-small intensity aerobic exercise, the rats heart had a good adaptive change, and the proteome of left ventricular muscle changed significantly. Long-term moderate-small intensity aerobic exercise can effective enhance the ability of myocardial antioxidation.
目的:探讨长期中小强度有氧运动对大鼠左室肌蛋白质组差异性表达的影响,筛选出对中小强度有氧运动刺激敏感的目标蛋白质,丰富运动健身的基础理论及为慢性心血管疾病的康复治疗提供新的思路和实验依据。方法:20只雄性SD大鼠随机分为运动组(E组)和对照组(C组)(n=10)。建立大鼠长期中小强度有氧运动跑台训练模型,采用双向凝胶电泳(2-DE)对大鼠左心室肌全蛋白样品进行分离。采用串联飞行时间质谱蛋白质仪对部分分离后差异表达量上调大于5倍或下调超过80%的蛋白质点进行质谱鉴定。结果:与C组比较,E组大鼠心脏重量指数(HWI)增加了32.0%,具有显著性差异(P＜0.05);与C组比较,E组有71个蛋白质点表达上调≥2倍或下调≥50%,对4个表达上调≥5倍或下调大于等于80%的蛋白质点进行质谱鉴定,鉴定出3个蛋白质和1个未知蛋白质。结论:长期中小强度有氧运动后,大鼠心脏发生了良好的适应性改变;大鼠左心室肌蛋白质组发生了明显变化,长期中小强度有氧运动能有效增强大鼠心肌抗氧化能力。.
Keywords: left-ventricular muscle; mass spectrometry identification; moderate-small intensity aerobic exercise; proteome; two dimensional gel electrophoresis.