Metabolomic and lipidomic characterization of an X-chromosome deletion disorder in neural progenitor cells by UHPLC-HRMS

Hoda Safari Yazd; Vanessa Y Rubio; Casey A Chamberlain; Richard A Yost; Timothy J Garrett

doi:10.1016/j.jmsacl.2021.05.002

Metabolomic and lipidomic characterization of an X-chromosome deletion disorder in neural progenitor cells by UHPLC-HRMS

J Mass Spectrom Adv Clin Lab. 2021 May 29:20:11-24. doi: 10.1016/j.jmsacl.2021.05.002. eCollection 2021 Apr.

Authors

Hoda Safari Yazd¹, Vanessa Y Rubio^{1

2}, Casey A Chamberlain^{3

4}, Richard A Yost¹, Timothy J Garrett³

Affiliations

¹ Department of Chemistry, University of Florida, Gainesville, FL 32610, USA.
² Department of Chemistry & Biochemistry, University of North Carolina at Greensboro, Greensboro, NC 27412, USA.
³ Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA.
⁴ Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.

Abstract

Introduction: Intellectual disorders involving deletions of the X chromosome present a difficult task in the determination of a connection between symptoms and metabolites that could lead to treatment options. One specific disorder of X-chromosomal deletion, Fragile X syndrome, is the most frequently occurring of intellectual disabilities. Previous metabolomic studies have been limited to mouse models that may not have sufficiently revealed the full biochemical diversity of the disease in humans.

Objectives: The primary objective of this study was to elucidate the human biochemistry in X-chromosomal deletion disorders through metabolomic and lipidomic profiling, using cells from a X-deletion patient as a representative case.

Methods: Metabolomic and lipidomic analysis was performed by UHPLC-HRMS on neural progenitor (NP) cells isolated from an afflicted female patient versus normal neural progenitor cells.

Results: Results showed perturbations in several metabolic pathways, including those of arginine and proline, that significantly impact both neurotransmitter generation and overall brain function. Coincidently, dysregulation was observed for lipids involved in both cellular structure and membrane integrity. The trends of observed metabolomic changes, as well as lipidomic profiling from identified features, are discussed.

Conclusion: The lipidomic and metabolomic profiles of NP cell samples exhibited significant differentiation associated with partial deletion of the X chromosome. These findings suggest that rare X-chromosomal deletion disorders are not only a mental disorder limited to alterations in local neuronal functions, but are also metabolic diseases.

Keywords: BMP, Bis(monoacylglycero) phosphate; Cer-NS, Ceramide nonhydroxyfatty acid-sphingosines; Fragile X syndrome; GL, Glycerolipid; HexCer-NS, Hexosylceramide nonhydroxyfatty acid-sphingosines; LPC, Lysophosphatidylcholines; Lipidomics; Metabolomics; Microdeletion; PC, Phosphatidylcholine; PE, Phosphatidylethanolamine; PG, Phosphatidylglycerol; SM, Sphingomyelin; SP, Sphingolipid; ST, Sterol; Xq27.3-Xq28.