Lipidomic profiling reveals molecular modification of lipids in hepatopancreas of juvenile mud crab (Scylla paramamosain) fed with different dietary DHA/EPA ratios

Xuexi Wang; Min Jin; Xin Cheng; Xiaoying Hu; Mingming Zhao; Ye Yuan; Peng Sun; Lefei Jiao; Douglas R Tocher; Mónica B Betancor; Qicun Zhou

doi:10.1016/j.foodchem.2021.131289

Lipidomic profiling reveals molecular modification of lipids in hepatopancreas of juvenile mud crab (Scylla paramamosain) fed with different dietary DHA/EPA ratios

Food Chem. 2022 Mar 15:372:131289. doi: 10.1016/j.foodchem.2021.131289. Epub 2021 Oct 6.

Authors

Xuexi Wang¹, Min Jin², Xin Cheng¹, Xiaoying Hu¹, Mingming Zhao¹, Ye Yuan¹, Peng Sun¹, Lefei Jiao¹, Douglas R Tocher³, Mónica B Betancor⁴, Qicun Zhou⁵

Affiliations

¹ Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China.
² Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China. Electronic address: jinmin@nbu.edu.cn.
³ Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou 515063, China.
⁴ Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.
⁵ Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China. Electronic address: zhouqicun@nbu.edu.cn.

PMID: 34818734
DOI: 10.1016/j.foodchem.2021.131289

Abstract

Untargeted lipidomic analysis was conducted to explore how different dietary docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) ratio and, specifically, how an optimal ratio (2.3) compared to a suboptimum ratio (0.6) impacted lipid molecular species and the positional distribution of fatty acids in hepatopancreas of mud crab. The results indicated that major category of lipid affected by dietary DHA/EPA ratio was glycerophospholipids (GPs). The optimum dietary DHA/EPA ratio increased the contents of DHA bound to the sn-2 and sn-3 positions of phosphatidylcholine (PC) and triacylglycerol, EPA bound to the sn-2 position of phosphatidylcholine and 18:2n-6 bound to the sn-2 position of phosphatidylethanolamine (PE). Increased dietary DHA/EPA ratio also led to competition between arachidonic acid (ARA) and 18:2n-6 bound to esterified sites. Appropriate dietary DHA/EPA ratio can not only improve the growth performance and nutritional quality of mud crab, but also provide higher quality products for human consumers.

Keywords: DHA/EPA; Fatty acid composition; Lipid molecules; Positional distribution; Scylla paramamosain; Untargeted lipidomics.

MeSH terms

Animals
Brachyura* / genetics
Docosahexaenoic Acids
Eicosapentaenoic Acid*
Hepatopancreas
Humans
Lipidomics

Substances

Docosahexaenoic Acids
Eicosapentaenoic Acid