Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

Janine T Bossé; Yanwen Li; Liza Miriam Cohen; Marc Stegger; Øystein Angen; Sonia Lacouture; Marcelo Gottschalk; Liancheng Lei; Miriam Koene; Peter Kuhnert; Aloka B Bandara; Thomas J Inzana; Matthew T G Holden; David Harris; Olusegun Oshota; Duncan J Maskell; Alexander W Tucker; Brendan W Wren; Andrew N Rycroft; Paul R Langford; On Behalf Of The BRaDP T Consortium

doi:10.1099/mgen.0.000687

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

Microb Genom. 2021 Nov;7(11):000687. doi: 10.1099/mgen.0.000687.

Authors

Janine T Bossé¹, Yanwen Li¹, Liza Miriam Cohen², Marc Stegger³, Øystein Angen³, Sonia Lacouture⁴, Marcelo Gottschalk⁴, Liancheng Lei⁵, Miriam Koene⁶, Peter Kuhnert⁷, Aloka B Bandara⁸, Thomas J Inzana^{9

8}, Matthew T G Holden^{10

11}, David Harris¹¹, Olusegun Oshota¹², Duncan J Maskell¹², Alexander W Tucker¹², Brendan W Wren¹³, Andrew N Rycroft¹⁴, Paul R Langford¹, On Behalf Of The BRaDP T Consortium

Affiliations

¹ Section of Paediatric Infectious Diseases, Department of Infectious Diseases, Imperial College London, London, UK.
² Department of Production Animal Clinical Sciences Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.
³ Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark.
⁴ Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Québec, Canada.
⁵ College of Veterinary Medicine, Jilin University, Changchun, P.R China.
⁶ Wageningen Bioveterinary Research, Lelystad, The Netherlands.
⁷ Institute of Veterinary Bacteriology, Vetsuisse Faculty, Universität Bern, Bern, Switzerland.
⁸ Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, USA.
⁹ Present address: College of Veterinary Medicine, Long Island University, Brookville, USA.
¹⁰ Present address: School of Medicine, University of St Andrews, St Andrews, UK.
¹¹ The Wellcome Trust Sanger Institute, Cambridge, UK.
¹² Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
¹³ Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
¹⁴ Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hatfield, UK.

Abstract

We report here the complete genome sequence of the widely studied Actinobacillus pleuropneumoniae serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the degS gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.

Keywords: Actinobacillus pleuropneumoniae; SNPs; serovar 8 reference genome.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Actinobacillus Infections*
Actinobacillus pleuropneumoniae* / classification
Actinobacillus pleuropneumoniae* / genetics
Animals
Genetic Variation
Genome, Bacterial
High-Throughput Nucleotide Sequencing
Serogroup
Swine
Swine Diseases*

Abstract

Publication types

MeSH terms

Grants and funding