Two-photon fluorogenic probe for visualizing PGP-1 activity in inflammatory tissues and serum from patients

Hua Bai; Bin Fang; Xujie Wang; Wenjing Qin; Yuhe Chen; Dongliang Zhang; Yan Li; Bo Peng; Xuekang Yang; Li Fu; Lin Li

doi:10.1039/d1cc05290j

Two-photon fluorogenic probe for visualizing PGP-1 activity in inflammatory tissues and serum from patients

Chem Commun (Camb). 2021 Dec 7;57(97):13186-13189. doi: 10.1039/d1cc05290j.

Authors

Hua Bai^{1

2}, Bin Fang^{1

3}, Xujie Wang⁴, Wenjing Qin⁵, Yuhe Chen¹, Dongliang Zhang⁴, Yan Li⁴, Bo Peng^{1

2}, Xuekang Yang⁴, Li Fu³, Lin Li¹

Affiliations

¹ Frontiers Science Center for Flexible Electronics, Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials & Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an 710072, P. R. China. iamlli@nwpu.edu.cn.
² Ningbo Institute of Northwestern Polytechnical University, Ningbo 315103, China.
³ State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an 710072, P. R. China. fuli@nwpu.edu.cn.
⁴ Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, P. R. China. yangxuekangburns@163.com.
⁵ Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), Nanjing, 211816, China.

PMID: 34816269
DOI: 10.1039/d1cc05290j

Abstract

A PGP-1-specific one/two-photon fluorogenic probe (BH1), capable of high sensitivity, super selectivity, and visual imaging of endogenous PGP-1 activity from live mammalian cells and serum/skin tissues from patients by using one/two-photon fluorescence microscopy (O/TPFM).

MeSH terms

Animals
Cell Line
Fluorescent Dyes / chemistry*
Humans
Inflammation / enzymology*
Inflammation / pathology
Mice
Microscopy, Fluorescence
Photons*
Pyroglutamyl-Peptidase I / blood
Pyroglutamyl-Peptidase I / metabolism*

Substances

Fluorescent Dyes
Pyroglutamyl-Peptidase I