Genetic characterization of co-trimoxazole non-susceptible Streptococcus pneumoniae isolates from Indonesia

Dodi Safari; Hanifah Fajri Maharani Putri; Arrayan Bimantari; Wisiva Tofriska Paramaiswari; Wisnu Tafroji; Miftahuddin Majid Khoeri; Korrie Salsabila

doi:10.1099/acmi.0.000271

Genetic characterization of co-trimoxazole non-susceptible Streptococcus pneumoniae isolates from Indonesia

Access Microbiol. 2021 Oct 25;3(10):000271. doi: 10.1099/acmi.0.000271. eCollection 2021.

Authors

Dodi Safari¹, Hanifah Fajri Maharani Putri^{1

2}, Arrayan Bimantari³, Wisiva Tofriska Paramaiswari¹, Wisnu Tafroji¹, Miftahuddin Majid Khoeri¹, Korrie Salsabila¹

Affiliations

¹ Eijkman Institute for Molecular Biology, Jakarta, Indonesia.
² Biology Program, Surya University, Tangerang, Indonesia.
³ Faculty of Biology, Gajah Mada University, Jogjakarta, Indonesia.

Abstract

We investigated the genetic variation of folA and folP genes encoding dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR) enzymes amongst trimethoprim/sulfamethoxazole (co-trimoxazole) non-susceptible Streptococcus pneumoniae isolated from the Indonesian population. Archived S. pneumoniae isolates were screened for the presence and analysis of folA and folP genes using the polymerase chain reaction sequencing method. We found that 80 % of co-trimoxazole non-susceptible isolates (n=30/39) showed a 6 bp insertion in the sulphonamide-binding site of DHPS. The Asp-92-Ala and Ile-100-Leu substitutions were more common on DHFR (42 %; 22/53) followed by the Asp-92-Ala, Glu-94-Asp and Ile-100-Leu substitutions (32 %; 17/53). The combination of the Ile-100-Leu substitution at the DHFR region and the 6 bp insertion was the most dominant combination among isolates having both folA and folP genes.

Keywords: Streptocococcus pneumoniae; co-trimoxazole; dihydrofolate reductase; dihydropteroate synthase.