Decellularised human bone allograft from different anatomical sites as a basis for functionally stratified repair material for bone defects

Halina T Norbertczak; Hazel L Fermor; Jennifer H Edwards; Paul Rooney; Eileen Ingham; Anthony Herbert

doi:10.1016/j.jmbbm.2021.104965

Decellularised human bone allograft from different anatomical sites as a basis for functionally stratified repair material for bone defects

J Mech Behav Biomed Mater. 2022 Jan:125:104965. doi: 10.1016/j.jmbbm.2021.104965. Epub 2021 Nov 13.

Authors

Halina T Norbertczak¹, Hazel L Fermor², Jennifer H Edwards², Paul Rooney³, Eileen Ingham², Anthony Herbert⁴

Affiliations

¹ Institute of Medical and Biological Engineering, School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom. Electronic address: h.t.norbertczak@leeds.ac.uk.
² Institute of Medical and Biological Engineering, School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom.
³ NHS Blood and Transplant Tissue and Eye Services, Liverpool, United Kingdom.
⁴ Institute of Medical and Biological Engineering, School of Mechanical Engineering, Faculty of Engineering and Physical Sciences, University of Leeds, Leeds, United Kingdom.

PMID: 34808451
DOI: 10.1016/j.jmbbm.2021.104965

Abstract

Tissue engineered bone solutions aim to overcome the limitations of autologous and allogeneic grafts. Decellularised tissues are produced by washing cellular components from human or animal tissue to produce an immunologically safe and biocompatible scaffold, capable of integration following implantation. A decellularisation procedure utilising low concentration sodium dodecyl sulphate (0.1% w/v) was applied to trabecular bone from human femoral heads (FH) and tibial plateaus (TP). Biological (histology, DNA quantification), biomechanical (compression testing) and structural (μCT) comparisons were made between decellularised and unprocessed cellular tissue. Total DNA levels of decellularised FH and TP bone were below 50 ng mg^-1 dry tissue weight and nuclear material was removed. No differences were found between cellular and decellularised bone, from each anatomical region, for all the biomechanical and structural parameters investigated. Differences were found between cellular FH and TP and between decellularised FH and TP. Decellularised FH had a higher ultimate compressive stress, Young's modulus and 0.2% proof stress than decellularised TP (p = 0.001, 0.002, 0.001, Mann Whitney U test, MWU). The mineral density of cellular and decellularised TP bone was significantly greater than cellular and decellularised FH bone respectively (cellular: p = 0.001, decellularised: p < 0.001, MWU). The bone volume fraction and trabecular thickness of cellular and decellularised FH bone were significantly greater than cellular and decellularised TP bone respectively (cellular: p = 0.001, 0.005; decellularised: p < 0.001, <0.001, MWU). Characterisation of decellularised trabecular bone from different anatomical regions offers the possibility of product stratification, allowing selection of biomechanical properties to match particular anatomical regions undergoing bone graft procedures.

Keywords: Decellularisation; Femoral head; Tibial plateau; Tissue scaffolds; Trabecular bone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acrylic Resins
Allografts
Animals
Bone Transplantation*
Humans

Substances

Acrylic Resins
Repair Material