gga-miR-200b-3p promotes avian leukosis virus subgroup J replication via targeting dual-specificity phosphatase 1

Yanling Pang; Yiming Yan; Xinheng Zhang; Feng Chen; Qingbin Luo; Qingmei Xie; Wencheng Lin

doi:10.1016/j.vetmic.2021.109278

gga-miR-200b-3p promotes avian leukosis virus subgroup J replication via targeting dual-specificity phosphatase 1

Vet Microbiol. 2022 Jan:264:109278. doi: 10.1016/j.vetmic.2021.109278. Epub 2021 Nov 11.

Authors

Yanling Pang¹, Yiming Yan¹, Xinheng Zhang², Feng Chen², Qingbin Luo², Qingmei Xie³, Wencheng Lin⁴

Affiliations

¹ College of Animal Science, South China Agricultural University, Guangzhou, 510642, PR China.
² College of Animal Science, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, Guangdong 510642, PR China.
³ College of Animal Science, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, Guangdong 510642, PR China. Electronic address: qmx@scau.edu.cn.
⁴ College of Animal Science, South China Agricultural University, Guangzhou, 510642, PR China; Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou, Guangdong 510642, PR China. Electronic address: wenchenglin@scau.edu.cn.

PMID: 34808431
DOI: 10.1016/j.vetmic.2021.109278

Abstract

MicroRNAs (miRNAs) involved host-virus interaction, affecting the replication or pathogenesis of several viruses. Although avian leukosis virus subgroup J (ALV-J) has been one of the most studied avian viruses, the effects of various host miRNAs on ALV-J infection and its underlying molecular mechanisms are still unclear. Here, we reported that gga-miR-200b-3p acts as a positive host factor enhancing ALV-J replication. We found that gga-miR-200b-3p was increased in response to ALV-J infection in host cells, and that gga-miR-200b-3p effectively enhanced ALV-J replication via targeting host protein dual-specificity phosphatase 1 (DUSP1). Collectively, these findings highlight a crucial role of gga-miR-200b-3p in ALV-J replication.

Keywords: ALV-J; DUSP1; gga-miR-200b-3p.

MeSH terms

Animals
Avian Leukosis Virus* / enzymology
Avian Leukosis Virus* / genetics
Avian Leukosis* / pathology
Avian Leukosis* / virology
Chickens
Dual-Specificity Phosphatases* / metabolism
Host Microbial Interactions* / physiology
MicroRNAs* / genetics
MicroRNAs* / metabolism
Virus Replication* / genetics

Substances

MicroRNAs
Dual-Specificity Phosphatases