Advanced platelet-rich fibrin (A-PRF) has an impact on the initial healing of gingival regeneration after tooth extraction

Yu-Hao Liu; Masahiro To; Toshimitsu Okudera; Satoko Wada-Takahashi; Shun-Suke Takahashi; Chen-Yao Su; Masato Matsuo

doi:10.1016/j.job.2021.11.001

Advanced platelet-rich fibrin (A-PRF) has an impact on the initial healing of gingival regeneration after tooth extraction

J Oral Biosci. 2022 Mar;64(1):141-147. doi: 10.1016/j.job.2021.11.001. Epub 2021 Nov 19.

Authors

Yu-Hao Liu¹, Masahiro To², Toshimitsu Okudera¹, Satoko Wada-Takahashi², Shun-Suke Takahashi³, Chen-Yao Su⁴, Masato Matsuo⁵

Affiliations

¹ Department of Clinical Oral Anatomy, Kanagawa Dental University, 82, Inaoka, Yokosuka, Kanagawa, 238-8580, Japan.
² Department of Physiology, Kanagawa Dental University, 82, Inaoka, Yokosuka, Kanagawa, 238-8580, Japan.
³ Department of Pharmacology, Kanagawa Dental University, 82, Inaoka, Yokosuka, Kanagawa, 238-8580, Japan.
⁴ Department of Clinical Oral Anatomy, Kanagawa Dental University, 82, Inaoka, Yokosuka, Kanagawa, 238-8580, Japan; International Society of Blood Biomaterials (ISBB), Sec.2, Linong Street, Taipei, 112 Taiwan.
⁵ Department of Clinical Oral Anatomy, Kanagawa Dental University, 82, Inaoka, Yokosuka, Kanagawa, 238-8580, Japan. Electronic address: m.matsuo@kdu.ac.jp.

PMID: 34808363
DOI: 10.1016/j.job.2021.11.001

Abstract

Objectives: Platelet-rich fibrin (PRF) is widely used in wound healing because it contains several growth factors, including vascular endothelial growth factor (VEGF). In this study, we investigated the effects of advanced PRF (A-PRF) in early-stage gingival regeneration after tooth extraction.

Methods: Blood sample was collected from females beagle dogs (age: 12 months) before tooth extraction for A-PRF preparation. All animals were sacrificed by perfusion-fixation on postoperative days 1, 3, and 7. The upper jaws were prepared for hematoxylin and eosin staining and immunostaining (for CD34 and VEGF). The lower jaw samples were prepared for scanning electron microscope observations. Blood flow in the gingiva before and after surgery was measured using laser Doppler flowmetry.

Results: In the A-PRF group, a large number of microvessels were observed in the gingival tissue on postoperative day 1. The microvessels in the control group were fewer and sparse. Regarding the vascular resin cast, a large number of new blood vessels were observed on postoperative day 1 in the A-PRF group. A stronger CD34-positive signal was obtained around the blood vessels in the A-PRF group than in the control group. Further, a strong VEGF-positive signal was observed in the perivascular tissue in the A-PRF group. Gingival blood flow was significantly higher in the A-PRF group after surgery.

Conclusion: A-PRF had a positive impact on angiogenesis in the gingiva through the induction of VEGF expression. Thus, A-PRF may be beneficial for gingival tissue regeneration.

Keywords: Gingival tissue; Micro vasculature; PRF; Regeneration; VEGF.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dogs
Female
Gingiva / surgery
Platelet-Rich Fibrin*
Tooth Extraction
Vascular Endothelial Growth Factor A
Wound Healing

Substances

Vascular Endothelial Growth Factor A