Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

Mei Xu; Wenhao Zhang; Mengyang Geng; Yiding Zhao; Shengyi Sun; Qian Gao; Yan Liu; Ling Shuai

doi:10.1016/j.xpro.2021.100881

Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

STAR Protoc. 2021 Nov 5;2(4):100881. doi: 10.1016/j.xpro.2021.100881. eCollection 2021 Dec 17.

Authors

Mei Xu¹, Wenhao Zhang¹, Mengyang Geng¹, Yiding Zhao¹, Shengyi Sun¹, Qian Gao¹, Yan Liu², Ling Shuai^{1

3}

Affiliations

¹ State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
² Department of Obstetrics, Tianjin First Central Hospital, Nankai University, Tianjin 300192, China.
³ National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.

Abstract

Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).

Keywords: Cell Biology; Cell Differentiation; Developmental biology; Genetics; High Throughput Screening; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Differentiation
Embryonic Stem Cells / cytology*
Haploidy*
Mice
Trophoblasts / cytology*