Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells

Kiren Jackson; Ezhilarasan Devaraj; Thangavelu Lakshmi; Shanmugam Rajeshkumar; Kamal Dua; Dinesh Kumar Chellappan; Subramanian Raghunandhakumar

doi:10.6026/97320630016817

Cytotoxic potentials of silibinin assisted silver nanoparticles on human colorectal HT-29 cancer cells

Bioinformation. 2020 Nov 30;16(11):817-827. doi: 10.6026/97320630016817. eCollection 2020.

Authors

Kiren Jackson¹, Ezhilarasan Devaraj¹, Thangavelu Lakshmi¹, Shanmugam Rajeshkumar¹, Kamal Dua^{2

3}, Dinesh Kumar Chellappan⁴, Subramanian Raghunandhakumar¹

Affiliations

¹ Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India 600 077.
² Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW 2007, Australia.
³ School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle, NSW 2308, Australia.
⁴ Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia.

Abstract

It is of interest to study the cytotoxicity of silibinin assisted silver nanoparticles in human colorectal (HT-29) cancer cells. Silver nanoparticles were synthesized using silibinin as a reducing agent. The synthesized silibinin assisted silver nanoparticles ( SSNPs) were characterized and analyzed using a transmission electron microscope and spectrophotometer. The SSNPs synthesized in this study are spherical and their size ranges from 10 to 80 nm. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/mL) of SSNPs and cytotoxicity was evaluated. The apoptosis was using flow cytometry. p53 protein expression using western blot. SSNPs are induced a decrease in viability and increased concentration-dependent cytotoxicity in HT-29 cells. SSNPs treatment also caused apoptosis-related morphological changes. SSNPs treatments at 8 and 16 ng/ml showed a prominent apoptotic change i.e., 70.3% and 83.6% respectively, and decreased viability of HT-29 cells 20% and 11.2% respectively as compared to control cells. SSNPs treatments induced p53 expression in HT-29 cells. Data shows that SSNPs have the potential to induce apoptosis in colorectal cancer cells. This provides insights for the further evaluation of SSNPs in fighting colon cancer.

Keywords: apoptosis; colorectal cancer; silibinin; silver nanoparticles.