Production and viscosity of Xanthan Gum are increased by LED irradiation of X. campestris cultivated in medium containing produced water of the oil industry

Pedro J L Crugeira; Paulo F de Almeida; Igor C F Sampaio; Luiz G P Soares; David A Moraga Amador; Ifor D W Samuel; Saydulla Persheyev; Landulfo Silveira Jr; Antônio L B Pinheiro

doi:10.1016/j.jphotobiol.2021.112356

Production and viscosity of Xanthan Gum are increased by LED irradiation of X. campestris cultivated in medium containing produced water of the oil industry

J Photochem Photobiol B. 2022 Jan:226:112356. doi: 10.1016/j.jphotobiol.2021.112356. Epub 2021 Nov 12.

Authors

Pedro J L Crugeira¹, Paulo F de Almeida², Igor C F Sampaio³, Luiz G P Soares⁴, David A Moraga Amador⁵, Ifor D W Samuel⁶, Saydulla Persheyev⁷, Landulfo Silveira Jr⁸, Antônio L B Pinheiro⁹

Affiliations

¹ Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil; Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP: 40110-100, Brazil. Electronic address: pedro.crugeira@ipb.pt.
² Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP: 40110-100, Brazil. Electronic address: pfatk@yahoo.com.br.
³ Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP: 40110-100, Brazil. Electronic address: igorsampaio@outlook.com.
⁴ Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil. Electronic address: luizgpinheiro@gmail.com.
⁵ NextGen DNA Sequencing Scientific Director, UF-ICBR, Bldg CGRC, Room 178, 2033 Mowry Road, Gainesville, FL 32610, United States. Electronic address: moraga@ufl.edu.
⁶ University of St Andrews UK, School of Physics & Astronomy, Physical Science Building, North Haugh, St Andrews, Fife KY16 9SS, United Kingdom. Electronic address: idws@st-andrews.ac.uk.
⁷ University of St Andrews UK, School of Physics & Astronomy, Physical Science Building, North Haugh, St Andrews, Fife KY16 9SS, United Kingdom. Electronic address: sp95@st-andrews.ac.uk.
⁸ Center for Innovation, Technology and Education - CITE, Universidade Anhembi Morumbi - UAM, Estr. Dr. Altino Bondensan, 500, São José dos Campos, SP CEP: 12247-016, Brazil. Electronic address: landulfo.silveira@gmail.com.
⁹ Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil. Electronic address: albp@ufba.br.

PMID: 34801926
DOI: 10.1016/j.jphotobiol.2021.112356

Abstract

Oil recovery is a challenge and microbial enhanced oil recovery is an option. We theorized that the use of produced water (PW) with photo-stimulation could influence both production and viscosity of Xanthan gum. This study aimed at the evaluation of the effect of photo-stimulation by λ630 ± 1 ηm LED light on the biosynthesis of Xanthan gum produced by Xanthomonas campestris IBSBF 2103 strain reusing PW of the oil industry. We assessed the effect of photo-stimulation by LED light (λ630 nm) on the biosynthesis of Xanthan gum produced by X. campestris in medium containing produced water. Different energy densities applied during the microbial growth phase were tested. The highest production was achieved when using 12 J/cm² LED light (p < 0.01). Three protocols were assessed: Non-irradiated (Control), Irradiation with LED light during the growth phase (LED_growth) and Irradiation with LED light during both growth and production phases (LED _{growth+production}). Both the amount and viscosity of the xanthan gum was significantly higher (p < 0.01) in the group LED_{growth+production}. The study showed that LED irradiation (λ630 ± 1 ηm) during both the growth and production phases of the biopolymer increased both the production and viscosity of Xanthan gum.

Keywords: Biopolymer; MEOR; Photo-stimulation; Thermoplastic property.

MeSH terms

Viscosity*