Yeast metabolic engineering for carbon dioxide fixation and its application

Soo Rin Kim; Soo-Jung Kim; Sun-Ki Kim; Seung-Oh Seo; Sujeong Park; Jamin Shin; Jeong-Sun Kim; Bo-Ram Park; Yong-Su Jin; Pahn-Shick Chang; Yong-Cheol Park

doi:10.1016/j.biortech.2021.126349

Yeast metabolic engineering for carbon dioxide fixation and its application

Bioresour Technol. 2022 Feb:346:126349. doi: 10.1016/j.biortech.2021.126349. Epub 2021 Nov 17.

Authors

Soo Rin Kim¹, Soo-Jung Kim², Sun-Ki Kim³, Seung-Oh Seo⁴, Sujeong Park¹, Jamin Shin¹, Jeong-Sun Kim⁵, Bo-Ram Park⁶, Yong-Su Jin⁷, Pahn-Shick Chang⁸, Yong-Cheol Park⁹

Affiliations

¹ School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
² Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea.
³ Department of Food Science and Technology, Chung-Ang University, Anseong, Gyeonggi 17546, Republic of Korea.
⁴ Department of Food Science and Nutrition, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
⁵ Department of Chemistry, Chonnam National University, Gwangju 61186, Republic of Korea.
⁶ Department of Agro-food Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
⁷ Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
⁸ Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
⁹ Department of Bio and Fermentation Convergence Technology, Kookmin University, Seoul 02707, Republic of Korea. Electronic address: ycpark@kookmin.ac.kr.

PMID: 34800639
DOI: 10.1016/j.biortech.2021.126349

Abstract

As numerous industrial bioprocesses rely on yeast fermentation, developing CO₂-fixing yeast strains can be an attractive option toward sustainable industrial processes and carbon neutrality. Recent studies have shown that the expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) in yeasts, such as Saccharomyces cerevisiae and Kluyveromyces marxianus, enables mixotrophic CO₂ fixation and production of biofuels. Also, the expression of a synthetic Calvin-Benson-Bassham (CBB) cycle including RuBisCO in Pichia pastoris enables autotrophic growth on CO₂. This review highlights recent advances in metabolic engineering strategies to enable CO₂ fixation in yeasts. Also, we discuss the potentials of other natural and synthetic metabolic pathways independent of RuBisCO for developing CO₂-fixing yeast strains capable of producing value-added biochemicals.

Keywords: Calvin-Benson-Bassham (CBB) cycle; Carbon dioxide fixation; Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO); Yeast metabolic engineering.

Publication types

Review

MeSH terms

Carbon Cycle
Carbon Dioxide*
Metabolic Engineering*
Photosynthesis
Ribulose-Bisphosphate Carboxylase / metabolism
Saccharomyces cerevisiae / metabolism

Substances

Carbon Dioxide
Ribulose-Bisphosphate Carboxylase