Low-cost apparatus for cigarette smoke exposure in rats

Abstract

Background: The effects of tobacco smoke on the central nervous system are usually studied with isolated nicotine, ignoring other compounds present in cigarette smoke. The few studies that use in vivo whole-body cigarette smoke exposure are usually performed in expensive commercial apparatus.

New method: We presented a feasible, safe, and low-cost apparatus for cigarette smoke exposure in rodents.

Results: Rats exposed to cigarette smoke in this apparatus showed cotinine levels similar to human active smokers. Additional results showed that cigarette smoke exposure increased glutamate and aspartic acid levels and decreased leucine, isoleucine, ornithine, phenylalanine, and tryptophan levels in the cerebrospinal fluid of rats.

Comparison with existing method(s): Our apparatus is feasible, safe, and costs 67-fold less than a commercial automatized smoking machine. Beyond the low cost, it does not require specialized knowledge for building or maintenance.

Conclusions: We concluded that our low-cost apparatus is reliable and reproduces cigarette smoke use in humans.

Keywords: amino acid; aspartic acid; cerebrospinal fluid; glutamate; nicotine; tobacco.