The proliferation and colonization of functional bacteria on amorphous polyethylene terephthalate: Key role of ultraviolet irradiation and nonionic surfactant polysorbate 80 addition

Abstract

Environmental pollution with plastics including polyethylene terephthalate (PET) has become a severe global problem, especially microplastic pollution, which is acknowledged as an emerging global pollutant. Biodegradation as a feasible and promising method has been studied, while colonization as the initiating step of the degradation process has seldom been studied. Here in this paper, we explored for the first time the key role of ultraviolet (UV) irradiation and nonionic surfactant polysorbate 80 (Tween-80, 0.2% V/V) in the proliferation and colonization of three functional bacteria (Pseudomonas putida, Pseudomonas sp. and Paracoccus sp.) on amorphous PET (APET). We found that 25 days of UV irradiation can trigger photolytic degradation process (appear the stretching vibration of associating carboxyl end group and the in-plane bending vibration of -OH) and introduce oxygen-containing functional groups on the surface of APET, even though the hydrophobicity of APET was scarcely changed. With regard to Tween-80, it can be utilized by these bacteria strains as carbon source to promote the proliferation, and it can also improve the cell surface hydrophobicity to stimulate the bacterial colonization during the first ten days of the experiment. When UV-irradiation and Tween-80 were provided together, the former factor can provide the target sites for functional bacteria to colonize, and the later factor can provide more candidates waiting to colonize by stimulating proliferation. As a result, an even better proliferation and colonization result can be achieved through the synergistic effect between the two factors. To some extent, the exposure between potential degrading bacteria and substrates to be degraded can be increased, which will create conditions for degrading. Generally, this research can provide certain theoretical basis and technical guidance for the remediation of plastic-polluted soil and the ocean.

Keywords: Colonization; Functional bacteria; Polyethylene terephthalate; Polysorbate 80; Proliferation; Ultraviolet irradiation.