miR-21-5p inhibits inflammation injuries in LPS-treated H9c2 cells by regulating PDCD4

Jianhua Xue; Jiajia Liu; Bo Xu; Junbo Yu; Aixian Zhang; Lili Qin; Chun Liu; Yang Yang

miR-21-5p inhibits inflammation injuries in LPS-treated H9c2 cells by regulating PDCD4

Am J Transl Res. 2021 Oct 15;13(10):11450-11460. eCollection 2021.

Authors

Jianhua Xue¹, Jiajia Liu¹, Bo Xu², Junbo Yu¹, Aixian Zhang³, Lili Qin⁴, Chun Liu⁵, Yang Yang¹

Affiliations

¹ Department of Trauma Center, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, China.
² Department of Orthopaedics, Qidong Hospital of Traditional Chinese Medicine Nantong, Jiangsu Province, China.
³ Department of General Practice Medicine, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, China.
⁴ Department of Endoscopic Center, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, China.
⁵ Department of Emergency Medicine, Affiliated Hospital of Nantong University Nantong, Jiangsu Province, China.

PMID: 34786071
PMCID: PMC8581922

Abstract

Objectives: To explore the expression levels and the potential regulatory mechanism of miR-21-5p in LPS-treated H9c2 cells.

Methods: The secretions of the inflammatory cytokines induced by LPS in H9c2 cells were evaluated using ELISA. We used RT-RCR and western blot to measure the relative mRNA and protein expression levels in LPS-treated H9c2 cells. CCK-8 and EdU assays showed the viability and proliferation profiles of the H9c2 cells. TUNEL assays demonstrated the apoptotic behaviors of the H9c2 cells, and a luciferase reporter analysis was used to investigate the interactions between miR-21-5p and programmed cell death protein 4 (PDCD4).

Results: LPS induced damage to the H9c2 cells by reducing the cell viability and down-regulating miR-21-5p. On the other hand, miR-21-5p overexpression inhibited the LPS-induced inflammatory damage in the H9c2 cells. Moreover, PDCD4 was verified as a downstream target gene of miR-21-5p, and its expression was inhibited by the higher miR-21-5p content. Finally, miR-21-5p inhibited septic processes, and the PDCD4 overexpression rescued the miR-21-5p effect in the LPS-treated H9c2 cells.

Conclusion: Our findings suggest that miR-21-5p inhibits the LPS-induced progression of sepsis in H9c2 cells. Additionally, PDCD4 is a downstream target gene of miR-21-5p, and both molecules serve as potential therapeutic targets for heart sepsis patients.

Keywords: H9c2 cells; LPS; heart sepsis; miR-21-5p; programmed cell death protein 4.