Fabrication of MERS-nanovesicle biosensor composed of multi-functional DNA aptamer/graphene-MoS2 nanocomposite based on electrochemical and surface-enhanced Raman spectroscopy

Gahyeon Kim; Jinmyeong Kim; Soo Min Kim; Tatsuya Kato; Jinho Yoon; Seungwoo Noh; Enoch Y Park; Chulhwan Park; Taek Lee; Jeong-Woo Choi

doi:10.1016/j.snb.2021.131060

Fabrication of MERS-nanovesicle biosensor composed of multi-functional DNA aptamer/graphene-MoS₂ nanocomposite based on electrochemical and surface-enhanced Raman spectroscopy

Sens Actuators B Chem. 2022 Feb 1:352:131060. doi: 10.1016/j.snb.2021.131060. Epub 2021 Nov 6.

Authors

Affiliations

¹ Department of Chemical Engineering, Kwangwoon University, 20 Kwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea.
² Green Chemistry Research Division, Research Institute of Green Science and Technology Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, Japan.
³ Department of Chemical & Biomolecular Engineering, Sogang University, Baekbeom-Ro, Mapo-Gu, Seoul 04107, Republic of Korea.

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is one of the most harmful viruses for humans in nowadays. To prevent the spread of MERS-CoV, a valid detection method is highly needed. For the first time, a MERS-nanovesicle (NV) biosensor composed of multi-functional DNA aptamer and graphene oxide encapsulated molybdenum disulfide (GO-MoS₂) hybrid nanocomposite was fabricated based on electrochemical (EC) and surface-enhanced Raman spectroscopy (SERS) techniques. The MERS-NV aptamer was designed for specifically binding to the spike protein on MERS-NVs and it is prepared using the systematic evolution of ligands by exponential enrichment (SELEX) technique. For constructing a multi-functional MERS aptamer (MF-aptamer), the prepared aptamer was connected to the DNA 3-way junction (3WJ) structure. DNA 3WJ has the three arms that can connect the three individual functional groups including MERS aptamer (bioprobe), methylene blue (signal reporter) and thiol group (linker) Then, GO-MoS₂ hybrid nanocomposite was prepared for the substrate of EC/SERS-based MERS-NV biosensor construction. Then, the assembled multifunctional (MF) DNA aptamer was immobilized on GO-MoS₂. The proposed biosensor can detect MERS-NVs not only in a phosphate-buffered saline (PBS) solution (SERS LOD: 0.176 pg/ml, EIS LOD: 0.405 pg/ml) but also in diluted 10% saliva (SERS LOD: 0.525 pg/ml, EIS LOD: 0.645 pg/ml).

Keywords: Aptamer; MERS-NV; MERS-NV biosensor; SELEX; Surface-enhanced Raman spectroscopy.