iTRAQ-based quantitative proteomic analysis of Pelteobagrus vachelli liver in response to hypoxia

Abstract

Dissolved oxygen is one of the determinants in the healthy farming of Pelteobagrus vachelli. This study, we conducted quantitative proteomics on the juvenile P. vachelli livers using iTRAQ. P. vachelli were treated by 3.75 ± 0.25 mg O₂/L (hypoxia group) and 7.25 ± 0.25 mg O₂/L (control group) for 90 days. The results revealed that under hypoxic conditions, P. vachelli grew slower than control group. Proteomic profiling enabled us to identify 2618 proteins, of which 176 were significantly differentially abundant proteins (DAPs). Verification of protein regulation based on qRT-PCR indicated that the proteomics data were reliable. The top 20 significantly DAPs (10 up-regulated, 10 down-regulated) were primarily involved in energy metabolism, apoptosis inhibition, and heavy metal detoxification. KEGG pathway enrichment analysis revealed significant enrichment of 'protein digestion and absorption', 'glycolysis/gluconeogenesis', and 'phagosome'. Combining the proteomics results of short-term hypoxia (treated with 0.70 ± 0.10 mg O₂ /L for 4 h), we screened 36 common DAPs. The analysis of the 36 common DAPs indicated that P. vachelli responded to the hypoxia by regulating energy supply, inhibiting apoptosis, and disturbing defensive system. Our results lay a theoretical foundation for the cultivation of hypoxia-tolerant species and eco-breeding of P. vachelli. SIGNIFICANCE OF THE STUDY: The hypoxia tolerance of Pelteobagrus vachelli is poor, which will seriously lead to its death in high-density culture. This study analysed the liver proteome of P. vachelli under long-term hypoxia stress (treated for 90 days at 3.75 ± 0.25 mg O₂/L), and then combined the proteome results of short-term hypoxia stress (treated for 4 h at 0.70 ± 0.10 mg O₂/L). The results showed P. vachelli responded to the hypoxia by regulating energy supply, inhibiting apoptosis and disturbing defensive system. The study contributes to the breeding of new hypoxia-tolerant species of P. vachelli and lays the theoretical foundation for eco-breeding.

Keywords: Eco-breeding; Hypoxia stress; Liver; Pelteobagrus vachelli; Proteomics.