A spectroscopic probe for hypochlorous acid detection

Ning An; Dan Wang; Hui Zhao; Yunling Gao

doi:10.1016/j.saa.2021.120529

A spectroscopic probe for hypochlorous acid detection

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 15;267(Pt 2):120529. doi: 10.1016/j.saa.2021.120529. Epub 2021 Oct 26.

Authors

Ning An¹, Dan Wang¹, Hui Zhao¹, Yunling Gao²

Affiliations

¹ State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
² State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: gaoyl@zjut.edu.cn.

PMID: 34785148
DOI: 10.1016/j.saa.2021.120529

Abstract

A spectroscopic probe CMBT was synthesized and characterized. CMBT showed the specific recognition for HClO based on the turn-on blue fluorescence and naked-eye change from pink to colorless. NMR, IR, HRMS-ESI, and spectral analysis suggested that colorimetric and fluorescent change of CMBT to HClO originated from the conversion of CMBT to starting material coumarin-aldehyde 1 caused by the oxidization of HClO, which was responsible for the fluorescence recovery. The detection limit was calculated to be 1.61 μM and 6.58 μM for fluorescence and UV-vis analysis with a range up to 1 mM. HClO's fluorescence detection was successfully achieved in tap and river water samples. The prepared convenient paper test strips showed a distinct color change in varying concentrations of HClO. A multi-input molecular logic circuit was constructed.

Keywords: Colorimetric fluorescent probe; Hypochlorous; Logic circuit; Naked-eye recognition; Test paper.

MeSH terms

Colorimetry
Fluorescent Dyes*
Hypochlorous Acid*
Microscopy, Fluorescence
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Hypochlorous Acid