Mini CRISPR-Cas12f1: a new genome editing tool

Muhammad Jawad Akbar Awan; Imran Amin; Shahid Mansoor

doi:10.1016/j.tplants.2021.11.002

Mini CRISPR-Cas12f1: a new genome editing tool

Trends Plant Sci. 2022 Feb;27(2):110-112. doi: 10.1016/j.tplants.2021.11.002. Epub 2021 Nov 13.

Authors

Muhammad Jawad Akbar Awan¹, Imran Amin¹, Shahid Mansoor²

Affiliations

¹ Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Faisalabad, Pakistan.
² Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Constituent College of Pakistan Institute of Engineering and Applied Sciences, Faisalabad, Pakistan. Electronic address: shahidmansoor7@gmail.com.

PMID: 34785123
DOI: 10.1016/j.tplants.2021.11.002

Abstract

The clustered regularly interspaced short palindromic repeats and associated protein (CRISPR-Cas) toolbox enables targeted mutations to be introduced into a genome. However, the delivery of appropriately sized Cas effectors to develop transgene-free edited plants is a limiting factor. A novel mini CRISPR-Cas12f1 system recently reported by Wu et al. overcomes this challenge by deploying viral-based vectors and nanoparticles (NPs) as carriers.

Keywords: AsCas12f1; genome editing; nanoparticles; transgene free editing; viral vectors.

MeSH terms

CRISPR-Cas Systems / genetics
Gene Editing*
Nanoparticles*