Complete 1H and 13C NMR spectral assignment of d-glucofuranose

Abstract

Although d-glucose is the most common sugar in nature, only a few NMR studies have focused on its minor furanose forms, and they have been limited to the anomeric position. Here, complete ¹H and ¹³C NMR spectral analysis of α- and β-d-glucofuranose was performed, including signal assignment, chemical shifts, and coupling constants. Selective and non-selective 1D and 2D NMR experiments were used for the analysis, complemented by spin simulations and iterative spectral analysis.

Keywords: Coupling constants; Furanose; Glucofuranose; Glucose; NMR spectroscopy; Selective NMR experiment.