Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures

Jun Kawamoto; Tatsuo Kurihara

doi:10.1007/978-1-0716-1900-1_12

Membrane Vesicles Produced by Shewanella vesiculosa HM13 as a Prospective Platform for Secretory Production of Heterologous Proteins at Low Temperatures

Methods Mol Biol. 2022:2414:191-205. doi: 10.1007/978-1-0716-1900-1_12.

Authors

Jun Kawamoto¹, Tatsuo Kurihara²

Affiliations

¹ Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan.
² Institute for Chemical Research, Kyoto University, Uji, Kyoto, Japan. kurihara@scl.kyoto-u.ac.jp.

PMID: 34784039
DOI: 10.1007/978-1-0716-1900-1_12

Abstract

Extracellular membrane vesicles (EMVs) produced by Gram-negative bacteria are useful as a vaccine platform. During growth in broth at 18 °C, Shewanella vesiculosa HM13 produces a large number of EMVs that contain a 49-kDa major cargo protein, named P49. Enhanced green fluorescent protein fused to the C-terminus of P49 is delivered to EMVs, suggesting that P49 is useful as a carrier to target foreign proteins to EMVs for production of artificial EMVs with desired functions. This method is potentially useful for the preparation of designed vaccines and is described in detail in this chapter.

Keywords: Extracellular membrane vesicles; Protein fusion; Protein secretion; Protein transport; Recombinant protein production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cold Temperature
Extracellular Vesicles
Secretory Vesicles
Shewanella*

Supplementary concepts

Shewanella vesiculosa